质粒简介:
pJMP1质粒是一个枯草杆菌基因编辑质粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. PubMed Journal