pDGIEF枯草整合分泌表达质粒_枯草杆菌载体_质粒载体_U平台商城-普拉特泽生物

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pDGIEF枯草整合分泌表达质粒

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基本信息

产品名称： pDGIEF枯草整合分泌表达质粒
产品编号： P1790
规格： 10μl质粒/300μl菌液
原核抗性：氨苄青霉素Amp
克隆菌株：大肠杆菌DH5α
培养条件： 37℃
质粒简介： pDGIEF是一个枯草杆菌整合分泌表达质粒。
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LOCUS Exported 8717 bp ds-DNA circular SYN 10-AUG-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..8717

/organism="synthetic DNA construct"

/mol_type="other DNA"

CDS complement(823..1905)

/codon_start=1

/gene="lacI"

/product="lac repressor"

/label=lacI

/note="The lac repressor binds to the lac operator to

inhibit transcription in E. coli. This inhibition can be

relieved by adding lactose or

isopropyl-beta-D-thiogalactopyranoside (IPTG)."

/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL

NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV

EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH

EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA

MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC

YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR

ALADSLMQLARQVSRLESGQ"

protein_bind 2514..2530

/label=lac operator

/bound_moiety="lac repressor encoded by lacI"

/note="The lac repressor binds to the lac operator to

inhibit transcription in E. coli. This inhibition can be

relieved by adding lactose or

isopropyl-beta-D-thiogalactopyranoside (IPTG)."

rep_origin complement(6888..7476)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(7647..8507)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(8508..8612)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga

61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt

121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat

181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt

241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg

301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg

361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg

421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa

481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat

541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca

601 catggaaaca cacaaattaa aaactggtct gatcggatcc gcggctagcc atggtcgacg

661 cggccgcaaa attgaaaaaa tggtggaaac acttttttca atttttttgt tttattattt

721 aatatttggg aaatattcat tctaattggt aatcagattt tagaaaacaa taaacccttg

781 cataggggga tcggatccta actcacatta attgcgttgc gctcactgcc cgctttccag

841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt

901 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt

961 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag

1021 caggcgaaaa tcctgtttga tggtggttga cggcgggata taacatgagc tgtcttcggt

1081 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc

1141 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc

1201 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg

1261 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag

1321 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc

1381 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat

1441 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac

1501 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc

1561 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac

1621 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg

1681 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag

1741 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc

1801 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga

1861 gacaccggca tactctgcga catcgtataa cgttactggt ttcatcaaaa tcgtctccct

1921 ccgtttgaat atttgattga tcgtaaccag atgaagcact ctttccacta tccctacagt

1981 gttatggctt gaacaatcac gaaacaataa ttggtacgta cgatctttca gccgactcaa

2041 acatcaaatc ttacaaatgt agtctttgaa agtattacat atgtaagatt taaatgcaac

2101 cgttttttcg gaaggaaatg atgacctcgt ttccaccgga attagcttgc atgcctaccc

2161 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt

2221 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc

2281 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtaca

2341 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc

2401 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc

2461 gcccatatcg ggtacgtatc ggcttaccat acctgcttcc tccttaagct taattgttat

2521 ccgctcacaa ttccacacat tatgccacac cttgtagata aagtcaacaa ctttttgcaa

2581 aatttttcag gaattttagc agaggttgtt ctggatgtag aacaaaacat ctttccgctc

2641 ttgtgctgtt aggatatctt tcttggaagc taggtaggca agggctacct caaataaatc

2701 ttcttcaggg tgcgctattt ttaaggtgcc tagtgaggtc ttgaccactt cacccataat

2761 ttcagtgccg aatagtctgg actgggctgt gtagaattcc tgcagccctg gcgaatggcg

2821 attttcgttc gtgaatacat gttataataa ctataactaa taacgtaacg tgactggcaa

2881 gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg aaatgaaaga

2941 tcatatcata tataatctag aataaaatta actaaaataa ttattatcta gataaaaaat

3001 ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta acaactatgg

3061 atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca tacgaacaaa

3121 ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt ggtacttaca

3181 tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac tttttagtcg

3241 tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa attagaccta

3301 tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca attattattc

3361 agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat ggagaatggt

3421 tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca gatttaacca

3481 taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat gacttagagg

3541 aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat tcgtcagagg

3601 aattaataga taattatcag gatgatgaaa ccaactctat attaacttta tgccgtatga

3661 ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat gcagtggctg

3721 aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt tatcttggag

3781 agaatattga atggactaat gaaaatgtaa atttaactat aaactattta aataacagat

3841 taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat ttttttgttt

3901 tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta gaaaacaata

3961 aacccttgca tagggggatc tcgacatgga tgagcgatga tgatatccgt ttaggctggg

4021 cggtgatagc ttctcgttca ggcagtacgc ctcttttctt ttccagacct gagggaggcg

4081 gaaatggtgt gaggttcccg gggaaaagcc aaataggcga tcgcgggagt gctttatttg

4141 aagatcaggc tatcactgcg gtcaatagat ttcacaatgt gatggctgga cagcctgagg

4201 aactctcgaa cccgaatgga aacaaccaga tatttatgaa tcagcgcggc tcacatggcg

4261 ttgtgctggc aaatgcaggt tcatcctctg tctctatcaa tacggcaaca aaattgcctg

4321 atggcaggta tgacaataaa gctggagcgg gttcatttca agtgaacgat ggtaaactga

4381 caggcacgat caatgccagg tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc

4441 ctcatgtttt ccttgagaat tacaaaacag gtgtaacaca ttctttcaat gatcaactga

4501 cgattacctt gcgtgcagat gcgaatacaa caaaagccgt ttatcaaatc aataatggac

4561 cagacgacag gcgtttaagg atggagatca attcacaatc ggaaaaggag atccaatttg

4621 gcaaaacata caccatcatg ttaaaaggaa cgaacagtga tggtgtaacg aggaccgaga

4681 aatacagttt tgttaaaaga gatccagcgt cggccaaaac catcggctat caaaatccga

4741 atcattggag ccaggtaaat gcttatatct ataaacatga tgggagccga gtaattgaat

4801 tgaccggatc ttggcctgga aaaccaatga ctaaaaatgc agacggaatt tacacgctga

4861 cgctgcctgc ggacacggat acaaccaacg caaaagtgat ttttaataat ggcagcgccc

4921 aagtgcccgg tcagaatcag cctggctttg attacgtgct aaatggttta tataatgact

4981 cgggcttaag cggttctctt ccccattgag ggcaaggcta gacgggactt accgaaagaa

5041 accatcaatg atggtttctt ttttgttcat aaatcagaca aaacttttct cttgcaaaag

5101 tttgtgaagt gttgcacaat ataaatgtga aatacttcac aaacaaaaag acatcaaaga

5161 gaaacatacc ctgcaaggat gctgatattg tctgcatttg cgccggagca aaccaaaaac

5221 ctggtgagac acgccttgaa ttagtagaaa agaacttgaa gattttcaaa ggcatcgtta

5281 gtgaagtcat ggcgagcgga tttgacggca ttttcttagt cgcgacgcga ggctggatgg

5341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca

5401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc

5461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg

5521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc

5581 ccgcgttgcg tcgcggtgca tggagccggg ccacctactg aagtggattt ctttaagagc

5641 tccgataacc agaagcaata aaaaatcaaa tcggatttca ctatataatc tcactttatc

5701 taagatgaat ccgatggaag catcctgttt tctctcaatt tttttatcta aaacccagcg

5761 ttcgatgctt ctttgagcga acgatcaaaa ataagtgcct tcccatcaaa aaaatattct

5821 caacataaaa aactttgtgt aatacttgta acgctacatg gagattaact caatctagag

5881 ggtattaata atgatccaca gtagcgtaaa gcgttgggga aattcaccgg cggtgcggat

5941 cccggctacg ttaatgcagg cgctcaatct gaatattgat gatgaagtga agattgacct

6001 ggtggatggc aaattaatta ttgagccagt gcgtaaagag cccgtattta cgcttgctga

6061 actggtcaac gacatcacgc cggaaaacct ccacgagaat atcgactggg gagagccgaa

6121 agataaggaa gtctggtaaa tcgattatgt cttttgcgca gtcggcttaa accagttttc

6181 gctggtgcga aaaaagagtg tcttgtgaca cctaaattca aaatctatcg gt

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