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pcDNAFlagLamp2b-HA人源基因哺乳表达质粒

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基本信息

产品名称： pcDNAFlagLamp2b-HA人源基因哺乳表达质粒
产品编号： P1408
规格： 10μl质粒/300μl菌液
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LOCUS Exported 6880 bp ds-DNA circular SYN 26-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS .

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 6880)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..6880

/organism="synthetic DNA construct"

/mol_type="other DNA"

rep_origin complement(160..745)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(916..1776)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(1777..1881)

/gene="bla"

/label=AmpR promoter

enhancer 2147..2526

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 2527..2730

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

promoter 2775..2793

/label=T7 promoter

/note="promoter for bacteriophage T7 RNA polymerase"

misc_feature 2822..2905

/label=Lamp2 signal peptide

CDS 2906..2929

/codon_start=1

/product="FLAG(R) epitope tag, followed by an enterokinase

cleavage site"

/label=FLAG

/translation="DYKDDDDK"

misc_feature 2930..4093

/label=Lamp2b

CDS 4094..4120

/codon_start=1

/product="HA (human influenza hemagglutinin) epitope tag"

/label=HA

/translation="YPYDVPDYA"

polyA_signal 4223..4447

/label=bGH poly(A) signal

/note="bovine growth hormone polyadenylation signal"

rep_origin 4493..4921

/direction=RIGHT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 4935..5264

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 5115..5250

/label=SV40 ori

/note="SV40 origin of replication"

CDS 5313..6338

/codon_start=1

/gene="aph(4)-Ia"

/product="aminoglycoside phosphotransferase from E. coli"

/label=Hyg

/note="confers resistance to hygromycin"

/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

polyA_signal 6468..6589

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

ORIGIN

1 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat

61 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca

121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc

181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata

241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc

301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc

361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga

421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc

481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag

541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag

601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag

661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat

721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc

781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt

841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta

901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct

961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg

1021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga

1081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt

1141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt

1201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt

1261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat

1321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc

1381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc

1441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat

1501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag

1561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt

1621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc

1681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa

1741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg

1801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa

1861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc

1921 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag

1981 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa

2041 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt

2101 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg

2161 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc

2221 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca

2281 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt

2341 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg

2401 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag

2461 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt

2521 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg

2581 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa

2641 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt

2701 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac

2761 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gtgccgccgc

2821 catggtgtgc ttccgcctct tcccggttcc gggctcaggg ctcgttctgg tctgcctagt

2881 cctgggagct gtgcggtctt atgcagacta taaagatgac gatgacaagg gctcgagttt

2941 ggaacttaat ttgacagatt cagaaaatgc cacttgcctt tatgcaaaat ggcagatgaa

3001 tttcacagtt cgctatgaaa ctacaaataa aacttataaa actgtaacca tttcagacca

3061 tggcactgtg acatataatg gaagcatttg tggggatgat cagaatggtc ccaaaatagc

3121 agtgcagttc ggacctggct tttcctggat tgcgaatttt accaaggcag catctactta

3181 ttcaattgac agcgtctcat tttcctacaa cactggtgat aacacaacat ttcctgatgc

3241 tgaagataaa ggaattctta ctgttgatga acttttggcc atcagaattc cattgaatga

3301 cctttttaga tgcaatagtt tatcaacttt ggaaaagaat gatgttgtcc aacactactg

3361 ggatgttctt gtacaagctt ttgtccaaaa tggcacagtg agcacaaatg agttcctgtg

3421 tgataaagac aaaacttcaa cagtggcacc caccatacac accactgtgc catctcctac

3481 tacaacacct actccaaagg aaaaaccaga agctggaacc tattcagtta ataatggcaa

3541 tgatacttgc ctgctggcta ccatggggct gcagctgaac atcactcagg ataaggttgc

3601 ttcagttatt aacatcaacc ccaatacaac tcactccaca ggcagctgcc gttctcacac

3661 tgctctactt agactcaata gcagcactat taagtatcta gactttgtct ttgctgtgaa

3721 aaatgaaaac cgattttatc tgaaggaagt gaacatcagc atgtatttgg ttaatggctc

3781 cgttttcagc attgcaaata acaatctcag ctactgggat gcccccctgg gaagttctta

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