pCasSA金黄色葡萄球菌温敏编辑质粒_球菌系列质粒_质粒载体_U平台商城-普拉特泽生物

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pCasSA金黄色葡萄球菌温敏编辑质粒

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基本信息

产品名称： pCasSA金黄色葡萄球菌温敏编辑质粒
产品编号： P1813
规格： 10μl质粒/300μl菌液
原核抗性：卡那霉素Kan
筛选标记：氯霉素Chl
克隆菌株：大肠杆菌Stbl3
培养条件： 30℃
质粒简介： pCasSA是一个金黄色葡萄球菌温度敏感基因编辑质粒。
质粒图谱：

质粒序列：点击查看序列

LOCUS Exported 10239 bp ds-DNA circular SYN 07-JAN-2018

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..10239

/organism="synthetic DNA construct"

/mol_type="other DNA"

rep_origin 78..666

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

misc_RNA 804..879

/label=gRNA scaffold

/note="guide RNA scaffold for the Streptococcus pyogenes

CRISPR/Cas9 system"

CDS 1120..5226

/codon_start=1

/product="Cas9 (Csn1) endonuclease from the Streptococcus

pyogenes Type II CRISPR/Cas system"

/label=Cas9

/note="generates RNA-guided double strand breaks in DNA"

/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK

NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES

FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK

FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL

ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ

IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR

QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL

RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG

NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY

FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD

SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER

LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM

QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR

HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY

LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP

SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH

VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL

NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT

EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK

ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI

TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE

LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD

ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL

DATLIHQSITGLYETRIDLSQLGGD"

CDS 6793..7392

/codon_start=1

/label=repC

/translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV

VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY

MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM

DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"

CDS 8288..8938

/codon_start=1

/label=Chl

/translation="MKFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE

GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV

KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL

LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"

CDS 9311..10105

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/label=Kan

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

terminator 10136..10230

/label=lambda t0 terminator

/note="transcription terminator from phage lambda"

ORIGIN

1 gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa

61 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca

121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt

181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg

241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc

301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga

361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc

421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc

481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca

541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg

601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta

661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct

721 cacatgttct ttcctgcgtt gtcgacagag tttgcaaaat atacagggga ttatatataa

781 tggaaacgag accattggtc tcagttttag agctagaaat agcaagttaa aataaggcta

841 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgaga tctgtccata

901 cccatggtct agaatgctcg agtcagaaaa atatacctgt atcttttttc aaaagcaaac

961 atgctttggg taaacatgta ggtattaacg tcaatgcgac aatagtagca ttgattaaat

1021 gagaattagt aagtgtttta cttactaaat tttatttaac ctaaaaatga accacctgga

1081 tgtgtgggat taaaaagtga agagaggagg acatatcaca tggataagaa atactcaata

1141 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt

1201 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt

1261 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca

1321 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca

1381 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg

1441 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct

1501 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat

1561 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat

1621 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag

1681 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat

1741 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct

1801 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt

1861 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca

1921 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct

1981 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga

2041 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa

2101 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat

2161 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct

2221 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag

2281 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac

2341 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa

2401 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga

2461 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg

2521 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca

2581 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta

2641 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc

2701 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc

2761 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat

2821 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt

2881 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg

2941 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa

3001 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg

3061 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt

3121 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt

3181 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac

3241 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta

3301 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg

3361 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat

3421 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt

3481 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa

3541 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa

3601 ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcctt

3661 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg

3721 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta

3781 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt

3841 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc

3901 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat

3961 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga

4021 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg

4081 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag

4141 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa

4201 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa

4261 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg

4321 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg

4381 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag

4441 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat

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