pFastBac1-Gus昆虫杆状病毒胞内表达质粒_昆虫细胞载体_质粒载体_U平台商城-普拉特泽生物

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pFastBac1-Gus昆虫杆状病毒胞内表达质粒

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基本信息

产品名称： pFastBac1-Gus昆虫杆状病毒胞内表达质粒
产品编号： P1697
规格： 10μl质粒/300μl菌液
启动子： PH promoter
复制子： pUC
原核抗性：氨苄青霉素Amp
筛选标记：庆大霉素Gen
克隆菌株：大肠杆菌DH5α
培养条件： 37℃
质粒简介： pFastBac1-Gus是一个昆虫细胞杆状病毒胞内表达质粒。
质粒图谱：

质粒序列：点击查看序列

LOCUS Exported 6661 bp ds-DNA circular SYN 13-OCT-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..6661

/organism="synthetic DNA construct"

/mol_type="other DNA"

rep_origin 2..457

/direction=RIGHT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 484..588

/gene="bla"

/label=AmpR promoter

CDS 589..1449

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 1620..2208

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

mobile_element 2511..2735

/mobile_element_type="transposon:Tn7"

/note="mini-Tn7 element (right end of the Tn7 transposon)"

CDS complement(2802..3335)

/codon_start=1

/gene="aacC1"

/product="gentamycin acetyltransferase"

/label=GmR

/note="confers resistance to gentamycin"

/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD

LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS

EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR

EEVMHFDIDPSTAT"

promoter complement(3524..3552)

/gene="intI1 (promoter lies within the coding sequence)"

/label=Pc promoter

/note="class 1 integron promoter"

promoter 3905..3996

/gene="polh from Autographa californica"

/label=polyhedrin promoter

/note="promoter for the baculovirus polyhedrin gene"

CDS 4081..5892

/codon_start=1

/gene="uidA (or gusA) from E. coli"

/product="beta-glucuronidase"

/label=GUS

/translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI

AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV

MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD

FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ

VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF

LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW

ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD

KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD

LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY

TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK

PKSAAFLLQKRWTGMNFGEKPQQGGKQ"

polyA_signal 6152..6286

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

mobile_element 6315..6480

/mobile_element_type="transposon:Tn7"

/note="mini-Tn7 element (left end of the Tn7 transposon)"

ORIGIN

1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc

61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc

121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt

181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg

241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt

301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta

361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt

421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat

481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc

1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc

1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg

2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag

2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt

2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac

2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg

2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc

2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg

2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct

2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga

2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag

2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt

2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga

2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac

2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg

2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg

2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca

2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact

3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc

3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta

3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct

3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg

3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg

3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca

3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa

3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa

3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca

3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac

3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc

3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg

3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt

3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt

3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa

3901 taggatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt

3961 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc

4021 accatcgggc gcggatccat atatagggcc cgggttataa ttacctcagg tcgacgtccc

4081 atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca

4141 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa

4201 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt

4261 cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca

4321 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat

4381 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg

4441 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg

4501 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac

4561 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg

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