pCS2-nCas9n-nanos3’UTR鱼类基因编辑Cas9表达质粒_鱼类系列质粒_质粒载体_U平台商城-普拉特泽生物

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pCS2-nCas9n-nanos3’UTR鱼类基因编辑Cas9表达质粒

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基本信息

产品名称： pCS2-nCas9n-nanos3’UTR鱼类基因编辑Cas9表达质粒
产品编号： P1812
规格： 10μl质粒/300μl菌液
启动子： CMV IE94 promoter
原核抗性：氨苄青霉素Amp
克隆菌株：大肠杆菌Stbl3
培养条件： 37℃
表达宿主：斑马鱼
质粒简介： pCS2-nCas9n-nanos 3’UTR是一个鱼类基因编辑Cas9表达质粒。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 8906 bp ds-DNA circular SYN 29-DEC-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..8906

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 35..53

/label=SP6 promoter

/note="promoter for bacteriophage SP6 RNA polymerase"

regulatory 99..108

/regulatory_class="other"

/note="vertebrate consensus sequence for strong initiation

of translation (Kozak, 1987)"

CDS 117..137

/codon_start=1

/product="nuclear localization signal of SV40 (simian virus

40) large T antigen"

/label=SV40 NLS

/translation="PKKKRKV"

CDS 144..4244

/codon_start=1

/product="Cas9 (Csn1) endonuclease from the Streptococcus

pyogenes MGAS15252 Type II CRISPR/Cas system"

/label=Cas9

/note="generates RNA-guided double strand breaks in DNA"

/translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK

NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES

FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK

FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL

ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ

IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR

QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL

RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG

NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY

FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD

SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER

LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM

QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH

KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL

YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS

EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV

AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN

AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE

ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE

SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT

IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL

ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA

NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD

ATLIHQSITGLYETRIDLSQLGGD"

CDS 4260..4280

/codon_start=1

/product="nuclear localization signal of SV40 (simian virus

40) large T antigen"

/label=SV40 NLS

/translation="PKKKRKV"

misc_feature 4317..4932

/label=nanos 3'UTR

polyA_signal 4955..5089

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin complement(5643..6231)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(6402..7262)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(7263..7367)

/gene="bla"

/label=AmpR promoter

rep_origin complement(7393..7848)

/direction=LEFT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 7922..8906

/label=CMV IE94 promoter

/note="enhancer/promoter region of simian cytomegalovirus

major immediate early transcription unit IE94"

ORIGIN

1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag

61 ctacttgttc tttttgcagg atccgatcca atgtcgacgc caccatggct tctccaccta

121 agaagaagag aaaggtggga agcatggata agaagtatag catcggcctg gatattggaa

181 ctaactccgt gggttgggca gtgattacag acgactacaa ggtccctagc aagaaattta

241 aggtgctggg taacaccgac aggcacagca tcaagaaaaa tctgattgga gccctgctgt

301 tcggttctgg agagactgcc gaagcaacac gcctgaaaag aacagcaaga aggcgctata

361 ccagaaggaa gaatagaatc tgttacctgc aggagatttt ctctaacgaa atggctaagg

421 tggacgattc attctttcat aggctggagg aaagtttcct ggtcgaggaa gataagaaac

481 acgagcgcca tcctatcttt ggaaacattg tggacgaggt cgcctatcac gaaaaatacc

541 caaccatcta tcatctgcgc aagaaactgg ctgactctac tgataaagcc gacctgagac

601 tgatctatct ggctctggcc cacatgatta agttcagggg tcattttctg atcgagggcg

661 atctgaaccc cgacaattcc gatgtggaca agctgttcat ccagctggtc cagatttaca

721 atcagctgtt tgaggaaaac cctattaatg cttccagagt ggacgcaaaa gctatcctgt

781 cagccaggct gtccaagtca cgcagactgg agaacctgat tgcacagctg cccggagaaa

841 agaggaacgg tctgtttgga aatctgatcg ctctgagtct gggcctgact cctaacttca

901 aaagcaattt tgatctggct gaggacgcca aactgcagct gtcaaaggac acatatgacg

961 atgacctgga taacctgctg gcacagatcg gagatcagta cgctgacctg ttcctggctg

1021 ccaaaaatct gtccgacgca atcctgctgt cagatattct gagagtgaac agcgagatta

1081 caaaagctcc tctgagtgcc agcatgatca agagatatga cgagcaccat caggatctga

1141 ccctgctgaa ggctctggtc aggcagcagc tgccagagaa gtacaaggaa attttctttg

1201 atcagtccaa gaacggctac gccggttata tcgacggagg cgcatcacag gaggaattct

1261 acaagtttat caaacctatt ctggagaaga tggacggaac tgaggaactg ctggtgaaac

1321 tgaatagaga ggacctgctg aggaagcagc gcacatttga taacggttcc atcccacacc

1381 agattcatct gggagagctg cacgctatcc tgaggcgcca ggaagacttc tacccctttc

1441 tgaaagataa ccgcgagaag atcgaaaaaa ttctgacctt cagaatccct tactatgtgg

1501 gtccactggc tcgcggaaac agcagatttg cctggatgac tcgcaaatcc gaggaaacca

1561 ttactccttg gaacttcgag gaagtggtcg ataagggcgc ctctgcacag tccttcatcg

1621 agagaatgac taattttgac aaaaacctgc ccaatgagaa agtgctgcct aagcactccc

1681 tgctgtacga gtatttcact gtctataacg aactgacaaa ggtgaaatac gtcaccgagg

1741 gcatgagaaa gccagccttc ctgtcaggag agcagaagaa agcaatcgtg gatctgctgt

1801 ttaaaaccaa caggaaagtg actgtcaagc agctgaagga ggactacttc aagaaaattg

1861 aatgcttcga ttccgtggag atcagcggag tcgaagacag atttaacgca agcctgggcg

1921 cttaccacga tctgctgaag atcattaagg ataaagactt cctggacaac gaggaaaatg

1981 aggatatcct ggaagacatt gtgctgacac tgaccctgtt tgaggacaga ggaatgatcg

2041 aggaaagact gaaaacctat gctcatctgt tcgatgacaa ggtgatgaaa cagctgaaga

2101 gaaggcgcta cactggctgg ggtagactga gcaggaagct gatcaacggc attagggata

2161 aacagtcagg aaagacaatc ctggactttc tgaaaagtga tggcttcgcc aaccgcaatt

2221 ttatgcagct gattcacgat gacagtctga ccttcaaaga ggacatccag aaggctcagg

2281 tgtctggaca gggccactcc ctgcatgagc agattgcaaa cctggctgga agcccagcca

2341 tcaagaaagg cattctgcag acagtgaaaa tcgtcgatga gctggtgaaa gtcatgggcc

2401 ataagcccga aaacatcgtg attgagatgg ctcgcgaaaa tcagacaacc cagaagggtc

2461 agaagaacag tagagagagg atgaaaagaa tcgaggaagg cattaaggag ctgggtagcc

2521 agatcctgaa agagcaccca gtggaaaaca cacagctgca gaatgagaag ctgtatctgt

2581 actatctgca gaatggaaga gatatgtacg tggaccagga gctggatatt aacaggctgt

2641 ctgattacga cgtggatcat atcgtccccc agagtttcat caaagatgac agcattgaca

2701 acaaggtgct gaccaggtcc gacaaaaaca gaggaaaatc agataatgtc cctagtgagg

2761 aagtggtcaa gaaaatgaag aactactgga gacagctgct gaatgccaaa ctgatcactc

2821 agaggaagtt tgataacctg acaaaagcag agcgcggtgg actgtcagaa ctggacaaag

2881 ctggattcat caagaggcag ctggtggaaa cacgccagat cactaaacac gtcgcacaga

2941 ttctggatag tcgcatgaac acaaagtacg atgagaatga caaactgatc agagaagtga

3001 aggtcattac cctgaagagt aaactggtca gcgactttag gaaagatttc cagttttata

3061 aggtccgcga gattaacaat tatcaccatg cccatgacgc atacctgaac gccgtggtcg

3121 gtaccgcact gatcaagaaa tacccaaaac tggagagcga attcgtgtac ggagactata

3181 aggtgtacga tgtcagaaaa atgatcgcca agtccgagca ggaaattgga aaagctactg

3241 ccaagtattt cttttactca aacatcatga atttctttaa gacagagatc accctggcca

3301 atggagaaat ccgcaaaagg cccctgattg agacaaacgg agagacaggc gaaatcgtgt

3361 gggacaaagg cagagatttt gcaaccgtga ggaaggtcct gagcatgcct caagtgaata

3421 tcgtcaagaa aactgaggtg cagacaggcg gtttctcaaa agaaagtatt ctgccaaaac

3481 gcaactctga taagctgatc gctagaaaga aagactggga ccctaagaag tatggaggct

&nb