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pMA0911

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基本信息

产品名称： pMA0911
产品编号： P1420
规格： 10μl质粒/300μl菌液
复制子： pUC ori，F1 ori
质粒分类：枯草杆菌质粒；枯草游离质粒；枯草胞内质粒
质粒大小： 7215bp
原核抗性：氨苄青霉素Amp
5测序引物： PMA0911F：gcgaaaatgcctcacatttgtgcc
3测序引物： PMA0911R：cgggatctcagatctggtacgtacc
质粒简介： pMA0911是一个枯草杆菌胞内表达质粒。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 7215 bp ds-DNA circular SYN 10-AUG-2017

DEFINITION synthetic circular DNA

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 7215)

TITLE Direct Submission

JOURNAL Exported Sep 15, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..7215

/organism="synthetic DNA construct"

/mol_type="other DNA"

CDS 4..113

/codon_start=1

/label=Lip A SP

/translation="ILIWSASAAASKRMSMT*ISLLF*FLLNKRKS*HRR"

primer_bind complement(185..209)

/label=PMA0911R

rep_origin 537..992

/direction=RIGHT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 1018..1122

/gene="bla"

/label=AmpR promoter

CDS 1123..1419

/codon_start=1

/label=Amp

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

DCNLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSAE"

CDS 1466..1984

/codon_start=1

/label=Amp

/translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR

LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL

LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE

IGASLIKHW"

rep_origin 2155..2746

/direction=RIGHT

/label=ori

/note="high-copy-number colE1/pMB1/pBR322/pUC origin of

replication"

promoter complement(3516..3618)

/label=cat promoter

/note="promoter of the E. coli cat gene"

CDS 4157..5161

/codon_start=1

/label=rep

/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY

FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI

QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV

GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN

VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS

YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"

CDS 5321..6091

/codon_start=1

/label=Kana Neo

/translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR

QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG

QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT

FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD

SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"

CDS 6314..6712

/codon_start=1

/label=bleomycin

/translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI

HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL

KDQWWDERDFAVIDPDNNLISFFQQIKS"

primer_bind 7172..7195

/label=PMA0911F

ORIGIN

1 catatcctca tatggtcagc atccgccgca gcttcgaagc gtatgtcgat gacatgaata

61 tcattactgt tctgattcct gctgaacaaa aggaaatcat gacaccgccg gaattcgtcg

121 acaagcttgg taccagatct gatatccctg cagggatcct ctagagtcga gctcaagcta

181 gcttggtacg taccagatct gagatcccgg gttctagagg tcgaaattca cctcgaaagc

241 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc

301 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa

361 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa

421 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct

481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc

541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac

601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt

661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc

721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc

781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact

841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg

901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc

961 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc

1021 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

1081 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc

1141 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa

1201 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgac

1261 tgcaatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg

1321 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa

1381 gagcaactcg gtcgccgcat acactattct gcagaatgac ttggttgagt actcaccagt

1441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac

1501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct

1561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga

1621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac

1681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat

1741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg

1801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc

1861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc

1921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg

1981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta

2041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg

2101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga

2161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt

2221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag

2281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa

2341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag

2401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca

2461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac

2521 cgaagtactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag

2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct

2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga

2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc

2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt

2821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg

2881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg

2941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc

3001 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc

3061 accccaggct ttacacttta tgcttccggc atattctcaa taaacccttt agggaaatag

3121 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa

3181 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg

3241 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat

3301 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc

3361 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctaaacggt ctggttatag

3421 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata

3481 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat

3541 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa

3601 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta

3661 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat

3721 tcgaagacga aagggcatcg cgcgcgggaa attcccggga gagctcgata tcgcatgcgg

3781 tacctctaga agaagcttgg agacaaggta aaggataaaa cagcacaatt ccaagaaaaa

3841 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa

3901 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc

3961 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat

4021 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt

4081 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgactaa

4141 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta

4201 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg

4261 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa

4321 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac

4381 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa

4441 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa

4501 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg

4561 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata

4621 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa

4681 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt

4741 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat

4801 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa

4861 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt

4921 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca

4981 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct

5041 tgatgacaca gaagaaggcg atttga