pLVML-3×FLAG-MCS-IRES-Puro慢病毒过表达质粒_病毒系列载体_质粒载体_U平台商城-普拉特泽生物

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pLVML-3×FLAG-MCS-IRES-Puro慢病毒过表达质粒

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基本信息

产品名称： pLVML-3×FLAG-MCS-IRES-Puro慢病毒过表达质粒
产品编号： P1676
规格： 10μl质粒/300μl菌液
原核抗性：氨苄青霉素Amp
筛选标记：嘌呤霉素Puro
克隆菌株：大肠杆菌Stbl3
培养条件： 37℃
质粒简介： pLVML-3×FLAG-MCS-IRES-Puro是一个慢病毒过表达质粒，本质粒由武汉淼灵生物科技有限公司构建并分享至淼灵质粒平台。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 8215 bp ds-DNA circular SYN 01-DEC-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..8215

/organism="synthetic DNA construct"

/mol_type="other DNA"

LTR 1..634

/label=3' LTR

/note="3' long terminal repeat (LTR) from HIV-1"

misc_feature 681..806

/label=HIV-1 Psi

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 1303..1536

/label=RRE

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

misc_feature 2028..2143

/label=cPPT/CTS

/note="central polypurine tract and central termination

sequence of HIV-1 (lacking the first T)"

enhancer 2201..2504

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 2505..2708

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

regulatory 2809..2818

/regulatory_class="other"

/note="vertebrate consensus sequence for strong initiation

of translation (Kozak, 1987)"

CDS 2818..2883

/codon_start=1

/product="three tandem FLAG(R) epitope tags, followed by an

enterokinase cleavage site"

/label=3xFLAG

/translation="DYKDHDGDYKDHDIDYKDDDDK"

misc_feature 2884..2915

/label=MCS

misc_feature 2918..3491

/label=IRES

/note="internal ribosome entry site (IRES) of the

encephalomyocarditis virus (EMCV)"

CDS 3524..4123

/codon_start=1

/gene="pac from Streptomyces alboniger"

/product="puromycin N-acetyltransferase"

/label=PuroR

/note="confers resistance to puromycin"

/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

misc_feature 4137..4725

/label=WPRE

/note="woodchuck hepatitis virus posttranscriptional

regulatory element"

LTR 4932..5565

/label=3' LTR

/note="3' long terminal repeat (LTR) from HIV-1"

rep_origin complement(6095..6683)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(6854..7714)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(7715..7819)

/gene="bla"

/label=AmpR promoter

polyA_signal 7867..8001

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

ORIGIN

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca

61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac

121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca

181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg

241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag

301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg

361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat

421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga

481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct

541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc

601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag

661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg

721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga

781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg

841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg

901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct

961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat

1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca

1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc

1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag

1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc

1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg

1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc

1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc

1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct

1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa

1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca

1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt

1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt

1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta

1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt

1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct

1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga

1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag

2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac

2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg

2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa

2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg

2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt

2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg

2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc

2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc

2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca

2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta

2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa

2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc

2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggac

2821 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac

2881 aagctcgaga ctagttctag agcggccgcg gatcccgccc ctctccctcc ccccccccta

2941 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt

3001 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga

3061 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg

3121 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt

3181 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat

3241 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg

3301 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg

3361 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt

3421 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa

3481 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc

3541 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc

3601 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt

3661 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt

3721 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc

3781 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca

3841 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc

3901 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc

3961 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg

4021

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