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pLJM1-FLAG

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基本信息

产品名称： pLJM1-FLAG
产品编号： P1430
规格： 10μl质粒/300μl菌液
启动子： CMV promoter
复制子： pUC Ori,F1 Ori,SV40 Ori
终止子： SV40 poly(A) signal
质粒分类：病毒系列质粒；慢病毒类质粒；病毒表达质粒
质粒大小： 7389bp
质粒标签： N-FLAG
原核抗性：氨苄青霉素Amp
筛选标记：嘌呤霉素Puro
克隆菌株：大肠杆菌Stbl3
培养条件： 37℃，有氧，LB
表达宿主： 293T等哺乳细胞
诱导方式：无需诱导
5测序引物： CMV-F：CGCAAATGGGCGGTAGGCGTG
3测序引物： Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒简介： pLJM1-FLAG质粒由武汉淼灵生物科技有限公司构建并分享至淼灵质粒平台。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 7389 bp ds-DNA circular SYN 20-SEP-2017

DEFINITION synthetic circular DNA

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 7389)

TITLE Direct Submission

JOURNAL Exported Sep 20, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..7389

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 131..334

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

CDS 375..398

/codon_start=1

/product="FLAG(R) epitope tag, followed by an enterokinase

cleavage site"

/label=FLAG

/translation="DYKDDDDK"

misc_feature 472..589

/label=cPPT/CTS

/note="central polypurine tract and central termination

sequence of HIV-1"

promoter 638..1148

/label=hPGK promoter

/note="human phosphoglycerate kinase 1 promoter"

CDS 1170..1769

/codon_start=1

/gene="pac from Streptomyces alboniger"

/product="puromycin N-acetyltransferase"

/label=PuroR

/note="confers resistance to puromycin"

/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

LTR 1897..2130

/label=3' LTR (Delta-U3)

/note="self-inactivating 3' long terminal repeat (LTR) from

HIV-1"

polyA_signal 2202..2323

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin 2363..2498

/label=SV40 ori

/note="SV40 origin of replication"

rep_origin 2705..3160

/direction=RIGHT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 3186..3290

/gene="bla"

/label=AmpR promoter

CDS 3291..4151

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 4322..4910

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

promoter 5379..5605

/label=RSV promoter

/note="Rous sarcoma virus enhancer/promoter"

LTR 5606..5786

/label=5' LTR (truncated)

/note="truncated 5' long terminal repeat (LTR) from HIV-1"

misc_feature 5833..5958

/label=HIV-1 Psi

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 6451..6684

/label=RRE

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

ORIGIN

1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc

61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc

121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc

181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa

241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag

301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta

361 gcgctaccgg tatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga

421 cgatatcttc gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag

481 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga

541 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta

601 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc

661 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca

721 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct

781 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag

841 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta

901 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg

961 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg

1021 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct

1081 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct

1141 ctccccaggg ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg

1201 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc

1261 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag

1321 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg

1381 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga

1441 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag

1501 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct

1561 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg

1621 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct

1681 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct

1741 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg

1801 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg

1861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac

1921 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct

1981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag

2041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac

2101 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt

2161 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt

2221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac

2281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc

2341 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc

2401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga

2461 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc

2521 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa

2581 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt

2641 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa

2701 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg

2761 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc

2821 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga

2881 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt

2941 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat

3001 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat

3061 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa

3121 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa

3181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca

3241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc

3301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc

3361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt

3421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt

3481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg

3541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact

3601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg

3661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga

3721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg

3781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa

3841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac

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