基本信息
- 产品名称： pIEFBPR
- 产品编号： P1421
- 规格： 10μl质粒/300μl菌液
- 复制子： ColE1 Ori
- 质粒分类： 枯草杆菌质粒；枯草整合质粒；枯草分泌质粒
- 质粒大小： 8965bp
- 原核抗性： 氨苄青霉素Amp
- 克隆菌株： DH5α等大肠杆菌
- 培养条件： 37℃，有氧，LB
- 质粒简介： pIEFBPR质粒是一个枯草整合型分泌载体，该质粒的参考文献：mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.
-
质粒图谱：
- 质粒序列： 点击查看序列
▍最近浏览过的商品
在线客服