pEGFP-KIF3b人源基因哺乳表达质粒_其他_质粒载体_U平台商城-普拉特泽生物

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pEGFP-KIF3b人源基因哺乳表达质粒

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基本信息

产品名称： pEGFP-KIF3b人源基因哺乳表达质粒
产品编号： P1768
规格： 10μl质粒/300μl菌液
启动子： CMV
复制子： pUC
终止子： bGH
原核抗性：卡那霉素Kan
筛选标记：遗传霉素G418
克隆菌株：大肠杆菌DH5α
培养条件： 37℃
质粒简介： pEGFP-KIF3b(NM_004798.3)是一个人源基因哺乳过表达质粒。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 6957 bp ds-DNA circular SYN 05-DEC-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..6957

/organism="synthetic DNA construct"

/mol_type="other DNA"

enhancer 61..364

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 365..568

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

regulatory 607..616

/regulatory_class="other"

/note="vertebrate consensus sequence for strong initiation

of translation (Kozak, 1987)"

CDS 613..1329

/codon_start=1

/product="enhanced GFP"

/label=EGFP

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

EFVTAAGITLGMDELYK"

CDS 1351..3594

/codon_start=1

/label=KIF3B

/translation="MSKLKSSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKN

PKGTAHEMPKTFTFDAVYDWNAKQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTY

TMEGIRGDPEKRGVIPNSFDHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQTKR

LELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVIT

IECSEVGLDGENHIRVGKLNLVDLAGSERQAKTGAQGERLKEATKINLSLSALGNVISA

LVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNI

KNKPRVNEDPKDALLREFQEEIARLKAQLEKRSIGRRKRREKRREGGGSGGGGEEEEEE

GEEGEEEGDDKDDYWREQQEKLEIEKRAIVEDHSLVAEEKMRLLKEKEKKMEDLRREKD

AAEMLGAKIKAMESKLLVGGKNIVDHTNEQQKILEQKRQEIAEQKRREREIQQQMESRD

EETLELKETYSSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNEL

TRELKLKHLIIENFIPLEEKSKIMNRAFFDEEEDHWKLHPITRLENQQMMKRPVSAVGY

KRPLSQHARMSMMIRPEARYRAENIVLLELDMPSRTTRDYEGPAIAPKVQAALDAALQD

EDEIQVDASSFESTANKKSKARPKSGRKSGSSSSSSGTPASQLYPQSRGLVPK"

polyA_signal 3745..3866

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin complement(3873..4328)

/direction=LEFT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 4355..4459

/gene="bla"

/label=AmpR promoter

promoter 4461..4818

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 4669..4804

/label=SV40 ori

/note="SV40 origin of replication"

CDS 4853..5647

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/label=NeoR/KanR

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

polyA_signal 5879..5926

/label=HSV TK poly(A) signal

/note="herpes simplex virus thymidine kinase

polyadenylation signal (Cole and Stacy, 1985)"

rep_origin 6255..6843

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg

661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc

721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg

781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc

841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag

901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag

961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac

1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac

1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc

1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg

1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc

1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag

1321 ctgtacaagt ccggactcag atctcgagct atgtcaaagt tgaaaagctc agagtcagtc

1381 agggtggtgg ttcgctgtcg gcccatgaat ggcaaggaaa aggctgcttc gtatgacaaa

1441 gtggtggatg tggatgttaa gctggggcag gtgtctgtga agaaccccaa agggacggcc

1501 catgaaatgc ccaagacctt cacctttgat gccgtctatg actggaatgc caagcagttt

1561 gaactgtacg atgagacgtt ccgaccactt gttgactctg tcctgcaagg tttcaatgga

1621 accatttttg cctatggaca aactgggaca ggaaaaacct acaccatgga aggaatccgt

1681 ggtgaccctg aaaaaagagg agtcattcct aactcatttg accatatctt cacccacatc

1741 tctcgatccc agaatcaaca atacctggtc agggcttctt acttagagat ctaccaggag

1801 gagatccgag atttgctctc aaaggatcag accaaaaggc ttgagctcaa agagaggcct

1861 gacacaggag tgtatgtgaa agacctgtct tcctttgtca ccaagagtgt gaaggagata

1921 gagcatgtga tgaatgtggg gaaccagaac cgttctgtcg gtgctaccaa catgaacgag

1981 cacagctcgc gttctcatgc aattttcgtt atcactattg agtgcagcga ggtgggcctc

2041 gatggtgaaa accacatccg tgtaggaaaa ttgaaccttg tagatcttgc tggcagcgaa

2101 cggcaagcca agaccggcgc acaaggggag agattaaaag aagctaccaa gatcaacctc

2161 tccctttccg ctttgggtaa tgtcatctct gctctagtgg acggcaaaag cactcacatt

2221 ccatatcggg actcaaagct taccaggctc ctccaagatt cccttggtgg caatgccaag

2281 actgtgatgg tggccaacgt ggggcctgcc tcttacaacg tagaagagac tctgaccact

2341 ctgcgatatg ccaaccgtgc caaaaacatt aagaacaaac caagggtcaa tgaggacccc

2401 aaggatgccc tccttcgaga attccaggaa gagattgctc ggctcaaggc ccagctggaa

2461 aaacggtcca ttggtaggag gaagaggcga gagaagcgga gggaaggtgg tggcagtggt

2521 gggggtgggg aagaggagga ggaggaggga gaagagggtg aggaggaagg ggatgataag

2581 gatgattact ggcgggaaca gcaagaaaaa ctggagattg agaagcgggc cattgtagag

2641 gatcacagct tggttgcaga ggagaagatg aggctgctga aggagaaaga gaaaaagatg

2701 gaggacctgc ggcgggagaa ggatgctgcc gagatgctgg gcgccaagat caaggccatg

2761 gagagtaagt tgcttgttgg aggaaaaaat atagtagatc atacgaatga acagcagaaa

2821 atcctggagc agaaacgaca ggaaattgca gagcagaaac gtcgagaaag agaaatccag

2881 caacagatgg aaagtcgaga tgaggagacc ttggaactta aagagacata cagctcattg

2941 cagcaagagg tggacatcaa gaccaaaaaa ctcaaaaagc tcttctccaa gcttcaggca

3001 gtgaaggctg agatccatga cctccaagaa gaacacatca aggagcgcca agagctagag

3061 cagactcaga atgagctcac cagggagctg aaactcaagc atcttattat agaaaacttt

3121 atccctctgg aagaaaaaag taaaattatg aatagagcct tctttgatga agaggaagat

3181 cattggaaac tacatcctat aaccagactg gagaaccagc agatgatgaa gcggccagtc

3241 tcagccgtgg gatataagag accattgagc cagcacgcaa gaatgtccat gatgattcgt

3301 ccagaggccc gatatagggc agaaaacatt gtgctgttag agctggacat gcccagccgg

3361 accaccagag actatgaggg tccagccatt gcccccaagg tccaggctgc attggatgcg

3421 gctctgcagg atgaagatga gatacaggtg gatgcatcat catttgaaag cactgcaaat

3481 aagaaatcca aggccaggcc taaaagtgga aggaagtcgg gatcctcctc ctcttcctca

3541 ggaacccctg catctcagct ttatccacag tctcgggggc tggttccaaa gtaagtcgac

3601 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca

3661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat

3721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa

3781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt

3841 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa

3901 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa

3961 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa

4021 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca

4081 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg

4141 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc

4201 ggcgaacgtg gcgagaaa

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