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1136-Desmoplakin-GFP人源基因哺乳表达质粒
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1136-Desmoplakin-GFP人源基因哺乳表达质粒
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基本信息
产品名称:
1136-Desmoplakin-GFP人源基因哺乳表达质粒
产品编号:
P1602
规格:
10μl质粒/300μl菌液
启动子:
CMV promoter
终止子:
SV40 poly(A) signal
质粒大小:
13661bp
质粒标签:
C-EGFP
原核抗性:
卡那霉素Kan
筛选标记:
新霉素Neo/G418
克隆菌株:
大肠杆菌DH5α
培养条件:
37℃,有氧,LB
质粒简介:
1136- Desmoplakin-GFP质粒是一个哺乳细胞过表达质粒,CMV启动子驱动Desmoplakin人源基因和EGFP绿色荧光蛋白融合表达。该质粒的参考文献是:Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.
质粒图谱:
质粒序列:
点击查看序列
LOCUS Exported 13661 bp ds-DNA circular SYN 25-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13661)
AUTHORS .
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..13661
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 704..982
/label=DP insert 5'region
misc_feature 983..9600
/label=Desmoplakin
CDS 9614..10333
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 10456..10577
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(10584..11039)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 11066..11170
/gene="bla"
/label=AmpR promoter
promoter 11172..11529
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 11380..11515
/label=SV40 ori
/note="SV40 origin of replication"
CDS 11564..12358
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 12590..12637
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 12966..13554
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc
661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac
721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg
781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta
841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat
901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc
961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac
1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg
1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc
1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga
1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt
1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca
1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca
1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta
1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac
1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg
1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc
1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg
1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga
1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca
1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga
1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc
1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag
1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac
2041 tctgcagacg cagtggagtt ggattcttca gatcaccaag tgcattgatg ttcatctgaa
2101 agaaaatgct gcctactttc agttttttga agaggcgcag tctactgaag catacctgaa
2161 ggggctccag gactccatca ggaagaagta cccctgcgac aagaacatgc ccctgcagca
2221 cctgctggaa cagatcaagg agctggagaa agaacgagag aaaatccttg aatacaagcg
2281 tcaggtgcag aacttggtaa acaagtctaa gaagattgta cagctgaagc ctcgtaaccc
2341 agactacaga agcaataaac ccattattct cagagctctc tgtgactaca aacaagatca
2401 gaaaatcgtg cataaggggg atgagtgtat cctgaaggac aacaacgagc gcagcaagtg
2461 gtacgtgacg ggcccgggag gcgttgacat gcttgttccc tctgtggggc tgatcatccc
2521 tcctccgaac ccactggccg tggacctctc ttgcaagatt gagcagtact acgaagccat
2581 cttggctctg tggaaccagc tctacatcaa catgaagagc ctggtgtcct ggcactactg
2641 catgattgac atagagaaga tcagggccat gacaatcgcc aagctgaaaa caatgcggca
2701 ggaagattac atgaagacga tagccgacct tgagttacat taccaagagt tcatcagaaa
2761 tagccaaggc tcagagatgt ttggagatga tgacaagcgg aaaatacagt ctcagttcac
2821 cgatgcccag aagcattacc agaccctggt cattcagctc cctggctatc cccagcacca
2881 gacagtgacc acaactgaaa tcactcatca tggaacctgc caagatgtca accataataa
2941 agtaattgaa accaacagag aaaatgacaa gcaagaaaca tggatgctga tggagctgca
3001 gaagattcgc aggcagatag agcactgcga gggcaggatg actctcaaaa acctccctct
3061 agcagaccag gggtcttctc accacatcac agtgaaaatt aacgagctta agagtgtgca
3121 gaatgattca caagcaattg ctgaggttct caaccagctt aaagatatgc ttgccaactt
3181 cagaggttct gaaaagtact gctatttaca gaatgaagta tttggactat ttcagaaact
3241 ggaaaatatc aatggtgtta cagatggcta cttaaatagc ttatgcacag taagggcact
3301 gctccaggct attctccaaa cagaagacat gttaaaggtt tatgaagcca ggctcactga
3361 ggaggaaact gtctgcctgg acctggataa agtggaagct taccgctgtg gactgaagaa
3421 aataaaaaat gacttgaact tgaagaagtc gttgttggcc actatgaaga cagaactaca
3481 gaaagcccag cagatccact ctcagacttc acagcagtat ccactttatg atctggactt
3541 gggcaagttc ggtgaaaaag tcacacagct gacagaccgc tggcaaagga tagataaaca
3601 gatcgacttt agattatggg acctggagaa acaaatcaag caattgagga attatcgtga
3661 taactatcag gctttctgca agtggctcta tgatgctaaa cgccgccagg attccttaga
3721 atccatgaaa tttggagatt ccaacacagt catgcggttt ttgaatgagc agaagaactt
3781 gcacagtgaa atatctggca aacgagacaa atcagaggaa gtacaaaaaa ttgctgaact
3841 ttgcgccaat tcaattaagg attatgagct ccagctggcc tcatacacct caggactgga
3901 aactctgctg aacataccta tcaagaggac catgattcag tccccttctg gggtgattct
3961 gcaagaggct gcagatgttc atgctcggta cattgaacta cttacaagat ctggagacta
4021 ttacaggttc ttaagtgaga tgctgaagag tttggaagat ctgaagctga aaaataccaa
4081 gatcgaagtt ttggaagagg agctcagact ggcccgagat gccaactcgg aaaactgtaa
4141 taagaacaaa ttcctggatc agaacctgca gaaataccag gcagagtgtt cccagttcaa
4201 agcgaagctt gcgagcctgg aggagctgaa gagacaggct gagctggatg ggaagtcggc
4261 taagcaaaat ctagacaagt gctacggcca aataaaagaa ctcaatgaga agatcacccg
4321 actgacttat gagattgaag atgaaaagag aagaagaaaa tctgtggaag acagatttga
4381 ccaacagaag aatgactatg accaactgca gaaagcaagg caatgtgaaa aggagaacct
4441 tggttggcag aaattagagt ctgagaaagc catcaaggag aaggagtacg agattgaaag
4501 gttgagggtt ctactgcagg aagaaggcac ccggaagaga gaatatgaaa atgagctggc
4561 aaaggtaaga aaccactata atgaggagat gagtaattta aggaacaagt atgaaacaga
4621 gattaacatt acgaagacca ccatcaagga gatatccatg caaaaagagg atgattccaa
4681 aaatcttaga aaccagcttg atagactttc aagggaaaat cgagatctga aggatgaaat
4741 tgtcaggctc aatgacagca tcttgcaggc cactgagcag cgaaggcgag ctgaagaaaa
4801 cgcccttcag caaaaggcct gtggctctga gataatgcag aagaagcagc atctggagat
4861 agaactgaag caggtcatgc agcagcgctc tgaggacaat gcccggcaca agcagtccct
4921 ggaggaggct gccaagacca ttcaggacaa aaataaggag atcgagagac tcaaagctga
4981 gtttcaggag gaggccaagc gccgctggga atatgaaaat gaactgagta aggtaagaaa
5041 caattatgat gaggagatca ttagcttaaa aaatcagttt gagaccgaga tcaacatcac
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