1136-Desmoplakin-GFP人源基因哺乳表达质粒_其他_质粒载体_U平台商城-普拉特泽生物

首页 > 质粒载体 > 其他 > 1136-Desmoplakin-GFP人源基因哺乳表达质粒

U平台

1136-Desmoplakin-GFP人源基因哺乳表达质粒

商城价：询价￥0元会员折扣

会员价格

会员等级	会员价格
注册用户	￥0元
vip	￥0元

最近售出0件（已有 1437 人关注）
购买此商品可使用：0积分

数量

- + 库存(载入中···)

加入购物车

在线咨询收藏

商品简介
购买记录
顾客评价()

基本信息

产品名称： 1136-Desmoplakin-GFP人源基因哺乳表达质粒
产品编号： P1602
规格： 10μl质粒/300μl菌液
启动子： CMV promoter
终止子： SV40 poly(A) signal
质粒大小： 13661bp
质粒标签： C-EGFP
原核抗性：卡那霉素Kan
筛选标记：新霉素Neo/G418
克隆菌株：大肠杆菌DH5α
培养条件： 37℃，有氧，LB
质粒简介： 1136- Desmoplakin-GFP质粒是一个哺乳细胞过表达质粒，CMV启动子驱动Desmoplakin人源基因和EGFP绿色荧光蛋白融合表达。该质粒的参考文献是：Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.
质粒图谱：

质粒序列：点击查看序列

LOCUS Exported 13661 bp ds-DNA circular SYN 25-OCT-2017

DEFINITION synthetic circular DNA

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 13661)

AUTHORS .

TITLE Direct Submission

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..13661

/organism="synthetic DNA construct"

/mol_type="other DNA"

enhancer 68..371

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 372..575

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

misc_feature 704..982

/label=DP insert 5'region

misc_feature 983..9600

/label=Desmoplakin

CDS 9614..10333

/codon_start=1

/product="enhanced GFP"

/label=EGFP

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

EFVTAAGITLGMDELYK"

polyA_signal 10456..10577

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin complement(10584..11039)

/direction=LEFT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 11066..11170

/gene="bla"

/label=AmpR promoter

promoter 11172..11529

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 11380..11515

/label=SV40 ori

/note="SV40 origin of replication"

CDS 11564..12358

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/label=NeoR/KanR

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

polyA_signal 12590..12637

/label=HSV TK poly(A) signal

/note="herpes simplex virus thymidine kinase

polyadenylation signal (Cole and Stacy, 1985)"

rep_origin 12966..13554

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg

61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc

121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt

181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc

241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg

301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg

361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact

421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa

481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta

541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct

601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc

661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac

721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg

781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta

841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat

901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc

961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac

1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg

1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc

1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga

1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt

1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca

1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca

1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta

1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac

1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg

1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc

1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg

1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga

1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca

1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga

1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc

1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag

1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac

2041 tctgcagacg cagtggagtt ggattcttca gatcaccaag tgcattgatg ttcatctgaa

2101 agaaaatgct gcctactttc agttttttga agaggcgcag tctactgaag catacctgaa

2161 ggggctccag gactccatca ggaagaagta cccctgcgac aagaacatgc ccctgcagca

2221 cctgctggaa cagatcaagg agctggagaa agaacgagag aaaatccttg aatacaagcg

2281 tcaggtgcag aacttggtaa acaagtctaa gaagattgta cagctgaagc ctcgtaaccc

2341 agactacaga agcaataaac ccattattct cagagctctc tgtgactaca aacaagatca

2401 gaaaatcgtg cataaggggg atgagtgtat cctgaaggac aacaacgagc gcagcaagtg

2461 gtacgtgacg ggcccgggag gcgttgacat gcttgttccc tctgtggggc tgatcatccc

2521 tcctccgaac ccactggccg tggacctctc ttgcaagatt gagcagtact acgaagccat

2581 cttggctctg tggaaccagc tctacatcaa catgaagagc ctggtgtcct ggcactactg

2641 catgattgac atagagaaga tcagggccat gacaatcgcc aagctgaaaa caatgcggca

2701 ggaagattac atgaagacga tagccgacct tgagttacat taccaagagt tcatcagaaa

2761 tagccaaggc tcagagatgt ttggagatga tgacaagcgg aaaatacagt ctcagttcac

2821 cgatgcccag aagcattacc agaccctggt cattcagctc cctggctatc cccagcacca

2881 gacagtgacc acaactgaaa tcactcatca tggaacctgc caagatgtca accataataa

2941 agtaattgaa accaacagag aaaatgacaa gcaagaaaca tggatgctga tggagctgca

3001 gaagattcgc aggcagatag agcactgcga gggcaggatg actctcaaaa acctccctct

3061 agcagaccag gggtcttctc accacatcac agtgaaaatt aacgagctta agagtgtgca

3121 gaatgattca caagcaattg ctgaggttct caaccagctt aaagatatgc ttgccaactt

3181 cagaggttct gaaaagtact gctatttaca gaatgaagta tttggactat ttcagaaact

3241 ggaaaatatc aatggtgtta cagatggcta cttaaatagc ttatgcacag taagggcact

3301 gctccaggct attctccaaa cagaagacat gttaaaggtt tatgaagcca ggctcactga

3361 ggaggaaact gtctgcctgg acctggataa agtggaagct taccgctgtg gactgaagaa

3421 aataaaaaat gacttgaact tgaagaagtc gttgttggcc actatgaaga cagaactaca

3481 gaaagcccag cagatccact ctcagacttc acagcagtat ccactttatg atctggactt

3541 gggcaagttc ggtgaaaaag tcacacagct gacagaccgc tggcaaagga tagataaaca

3601 gatcgacttt agattatggg acctggagaa acaaatcaag caattgagga attatcgtga

3661 taactatcag gctttctgca agtggctcta tgatgctaaa cgccgccagg attccttaga

3721 atccatgaaa tttggagatt ccaacacagt catgcggttt ttgaatgagc agaagaactt

3781 gcacagtgaa atatctggca aacgagacaa atcagaggaa gtacaaaaaa ttgctgaact

3841 ttgcgccaat tcaattaagg attatgagct ccagctggcc tcatacacct caggactgga

3901 aactctgctg aacataccta tcaagaggac catgattcag tccccttctg gggtgattct

3961 gcaagaggct gcagatgttc atgctcggta cattgaacta cttacaagat ctggagacta

4021 ttacaggttc ttaagtgaga tgctgaagag tttggaagat ctgaagctga aaaataccaa

4081 gatcgaagtt ttggaagagg agctcagact ggcccgagat gccaactcgg aaaactgtaa

4141 taagaacaaa ttcctggatc agaacctgca gaaataccag gcagagtgtt cccagttcaa

4201 agcgaagctt gcgagcctgg aggagctgaa gagacaggct gagctggatg ggaagtcggc

4261 taagcaaaat ctagacaagt gctacggcca aataaaagaa ctcaatgaga agatcacccg

4321 actgacttat gagattgaag atgaaaagag aagaagaaaa tctgtggaag acagatttga

4381 ccaacagaag aatgactatg accaactgca gaaagcaagg caatgtgaaa aggagaacct

4441 tggttggcag aaattagagt ctgagaaagc catcaaggag aaggagtacg agattgaaag

4501 gttgagggtt ctactgcagg aagaaggcac ccggaagaga gaatatgaaa atgagctggc

4561 aaaggtaaga aaccactata atgaggagat gagtaattta aggaacaagt atgaaacaga

4621 gattaacatt acgaagacca ccatcaagga gatatccatg caaaaagagg atgattccaa

4681 aaatcttaga aaccagcttg atagactttc aagggaaaat cgagatctga aggatgaaat

4741 tgtcaggctc aatgacagca tcttgcaggc cactgagcag cgaaggcgag ctgaagaaaa

4801 cgcccttcag caaaaggcct gtggctctga gataatgcag aagaagcagc atctggagat

4861 agaactgaag caggtcatgc agcagcgctc tgaggacaat gcccggcaca agcagtccct

4921 ggaggaggct gccaagacca ttcaggacaa aaataaggag atcgagagac tcaaagctga

4981 gtttcaggag gaggccaagc gccgctggga atatgaaaat gaactgagta aggtaagaaa

5041 caattatgat gaggagatca ttagcttaaa aaatcagttt gagaccgaga tcaacatcac

▍购买记录(近期成交数量0)

还没有人购买过此商品

总计 0 个记录，共 1 页。第一页上一页下一页最末页

▍用户评论(共0条评论)

暂时还没有任何用户评论

总计 0 个记录，共 1 页。上一页下一页

用户名：	匿名用户
E-mail：
评价等级：
评论内容：
验证码：

▍最近浏览过的商品

购物车(0)

联
系
客
服

U商城

订购指南
订购方式
售后服务
免责协议售后政策
关于我们
联系我们关于U商城

联系我们

客服热线：4006916686

在线客服

4006916686

会员价格

▍购买记录(近期成交数量0)

▍用户评论(共0条评论)

▍最近浏览过的商品

订购指南

售后服务

关于我们

联系我们

关注我们