E612,E619:E624_植物细胞载体_质粒载体_U平台商城-普拉特泽生物

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E612,E619:E624

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基本信息

产品名称： E612,E619:E624
产品编号： P1431
规格： 10μl质粒/300μl菌液
启动子： CaMV 35S promoter (enhanced)
复制子： pUC ori，pVS1 oriV
终止子： CaMV poly(A) signal
质粒分类：植物系列质粒；植物表达质粒；其它系列质粒
质粒大小： 10531bp
原核抗性：卡那霉素Kan
筛选标记：潮霉素Hyg
克隆菌株： DH5α等大肠杆菌
培养条件： 37℃，LB，有氧
表达宿主：植物
质粒简介： pLSLR是一个植物的T-DNA质粒，拥有潮霉素筛选标记。Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 10531 bp ds-DNA circular SYN 17-SEP-2017

DEFINITION synthetic circular DNA

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 10531)

TITLE Direct Submission

JOURNAL Exported Sep 17, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..10531

/organism="synthetic DNA construct"

/mol_type="other DNA"

rep_origin 1129..1323

/label=pVS1 oriV

/note="origin of replication for the Pseudomonas plasmid

pVS1 (Heeb et al., 2000)"

misc_feature 1667..1807

/label=bom

/note="basis of mobility region from pBR322"

rep_origin complement(1993..2581)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(2668..3462)

/codon_start=1

/gene="aphA-3"

/product="aminoglycoside phosphotransferase"

/label=KanR

/note="confers resistance to kanamycin"

/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

misc_feature 3887..3911

/label=LB T-DNA repeat

/note="left border repeat from nopaline C58 T-DNA"

polyA_signal 3989..4163

/label=CaMV poly(A) signal

/note="cauliflower mosaic virus polyadenylation signal"

CDS complement(4189..5331)

/codon_start=1

/label=Hyg

/translation="MTHNPTILRKTFLYIRKFISFGEDTLKSPVSLYKSISPRMKKPEL

TATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYR

HFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAA

ADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELML

WAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACM

EQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA

GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPGSWR"

promoter complement(5253..5930)

/label=CaMV 35S promoter (enhanced)

/note="cauliflower mosaic virus 35S promoter with a

duplicated enhancer region"

misc_feature 8140..8164

/label=RB T-DNA repeat

/note="right border repeat from nopaline C58 T-DNA"

CDS 9464..10093

/codon_start=1

/product="stability protein from the Pseudomonas plasmid

pVS1 (Heeb et al., 2000)"

/label=pVS1 StaA

/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

ORIGIN

1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt

61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc

121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc

181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct

241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc

301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga

361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc

421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg

481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg

541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat

601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt

661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga

721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt

781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg

841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg

901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga

961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg

1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct

1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac

1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa

1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga

1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc

1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct

1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat

1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga

1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc

1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca

1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc

1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt

1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg

1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg

1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa

1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc

1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc

2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag

2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct

2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta

2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc

2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc

2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt

2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct

2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc

2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca

2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta

2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt

2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc

2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc

2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt

2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa

2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc

3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct

3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata

3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc

3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt

3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata

3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac

3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc

3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct

3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca

3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca

3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca

3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca

3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc

3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg

3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt

3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt

3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta

4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc

4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact

4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga

4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc

4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga

4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac

4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga

4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa

4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc

4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa

4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc

4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac

4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg

4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat

4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt

4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc

4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt

5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa

5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa

5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg

5221 agag

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