pRGEB32_植物细胞载体_质粒载体_U平台商城-普拉特泽生物

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pRGEB32

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基本信息

产品名称： pRGEB32
产品编号： P1413
规格： 10μl质粒/300μl菌液
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LOCUS Exported 15888 bp ds-DNA circular SYN 24-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS .

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 15888)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 24, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..15888

/organism="synthetic DNA construct"

/mol_type="other DNA"

terminator 71..323

/label=NOS terminator

/note="nopaline synthase terminator and poly(A) signal"

misc_feature 551..575

/label=RB T-DNA repeat

/note="right border repeat from nopaline C58 T-DNA"

CDS 1876..2505

/codon_start=1

/product="stability protein from the Pseudomonas plasmid

pVS1 (Heeb et al., 2000)"

/label=pVS1 StaA

/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

CDS 2934..4007

/codon_start=1

/product="replication protein from the Pseudomonas plasmid

pVS1 (Heeb et al., 2000)"

/label=pVS1 RepA

/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW

QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS

KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP

GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE

ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR

LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI

LVMRYRNLIEGEASAGS"

rep_origin 4073..4267

/label=pVS1 oriV

/note="origin of replication for the Pseudomonas plasmid

pVS1 (Heeb et al., 2000)"

misc_feature 4611..4751

/label=bom

/note="basis of mobility region from pBR322"

rep_origin complement(4937..5525)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(5612..6406)

/codon_start=1

/gene="aphA-3"

/product="aminoglycoside phosphotransferase"

/label=KanR

/note="confers resistance to kanamycin"

/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

misc_feature 6831..6855

/label=LB T-DNA repeat

/note="left border repeat from nopaline C58 T-DNA"

polyA_signal 6933..7107

/label=CaMV poly(A) signal

/note="cauliflower mosaic virus polyadenylation signal"

CDS complement(7148..8173)

/codon_start=1

/gene="aph(4)-Ia"

/product="aminoglycoside phosphotransferase from E. coli"

/label=HygR

/note="confers resistance to hygromycin"

/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP

ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

K"

promoter complement(8240..8916)

/label=CaMV 35S promoter (enhanced)

/note="cauliflower mosaic virus 35S promoter with a

duplicated enhancer region"

promoter 9239..9619

/label=OsU3 promoter

/note="Oryza sativa (rice) snRNA U3 promoter"

misc_RNA 9636..9711

/label=gRNA scaffold

/note="guide RNA scaffold for the Streptococcus pyogenes

CRISPR/Cas9 system"

promoter 9792..11501

/gene="RUBQ2"

/label=Ubi promoter

/note="promoter for the rice (Oryza sativa) polyubiquitin

gene RUBQ2"

CDS 11581..11646

/codon_start=1

/product="three tandem FLAG(R) epitope tags, followed by an

enterokinase cleavage site"

/label=3xFLAG

/translation="DYKDHDGDYKDHDIDYKDDDDK"

CDS 11653..11673

/codon_start=1

/product="nuclear localization signal of SV40 (simian virus

40) large T antigen"

/label=SV40 NLS

/translation="PKKKRKV"

CDS 11698..15798

/codon_start=1

/product="Cas9 (Csn1) endonuclease from the Streptococcus

pyogenes Type II CRISPR/Cas system"

/label=Cas9

/note="generates RNA-guided double strand breaks in DNA"

/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN

LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF

LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF

RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE

NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI

GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ

QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR

KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN

SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF

TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS

VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL

KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ

LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH

KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL

YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS

EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV

AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN

AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE

ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE

SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT

IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL

ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA

NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD

ATLIHQSITGLYETRIDLSQLGGD"

CDS 15799..15846

/codon_start=1

/product="bipartite nuclear localization signal from

nucleoplasmin"

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验证码：

4006916686

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