ATU6-26-sgRNA-SK_植物细胞载体_质粒载体_U平台商城-普拉特泽生物

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ATU6-26-sgRNA-SK

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基本信息

产品名称： ATU6-26-sgRNA-SK
产品编号： P1410
规格： 10μl质粒/300μl菌液
质粒大小： 3760bp
原核抗性：氨苄青霉素Amp
质粒简介： ATU6-26-sgRNA-SK质粒
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 3760 bp ds-DNA circular SYN 26-SEP-2017

DEFINITION synthetic circular DNA

KEYWORDS ATU6-26-sgRNA-SK

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 3760)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..3760

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 64..168

/gene="bla"

/label=AmpR promoter

CDS 169..1029

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 1200..1789

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

misc_feature 2383..2389

/label=U6 terminator

misc_RNA complement(2390..2465)

/label=gRNA scaffold

/note="guide RNA scaffold for the Streptococcus pyogenes

CRISPR/Cas9 system"

misc_feature complement(2483..2931)

/label=AtU6-26 promoter

/note="Synthetic construct guide RNA, complete sequence

GenBank: KT827739.1"

rep_origin complement(3176..3631)

/direction=LEFT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

ORIGIN

1 ctgatgtcgc gcgcttatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat

61 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

121 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

241 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

301 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

361 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

421 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

481 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

541 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

601 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

661 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

721 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

781 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

841 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

901 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

961 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

1021 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

1081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

1141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

1201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

1261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

1321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc

1381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

1441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

1501 gcgcagcggt cgggctgaac gggggggttc gtgcacacag cccagcttgg agcgaacgac

1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg

1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga

1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact

1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa

1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc

1861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg

1921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat

1981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt

2041 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta

2101 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg

2161 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc

2221 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg

2281 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagcggcc

2341 gccagtgtga tggatatctg cagaattgcc cttcgaaggg acaaaaaaag caccgactcg

2401 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct

2461 aaaacaggtc ttctcgaaga cccaatcact acttcgactc tagctgtata taaactcagc

2521 ttcgttttct tatctaagcg atgtgggact tttgaagatt gttttcaact taaatgggcc

2581 tatataagaa atactattgt tctttcccat ataaatgggc ctgcttctct tctttcagat

2641 tcccaggggc cttttgaaga ttatcttcat atcttaagaa tgaagatgtt ttattcaatc

2701 aaattcttga aggttcgatg cctaatcatt ctaatcctgg gacaaactat gaaacaagat

2761 acaaaaactc cgaatggaaa gttaaaaaga agaaaacgaa agctacggtt caagaaaatg

2821 taagctgata aacaaaaaaa aactgtatga acgaagaaga agaaaaaaag ctaagaagaa

2881 atgatgtatt gtgcggaagg caagtcgagt ttccgttgtt caacgaagct taagggcaat

2941 tccagcacac tggcggccgt tactagtgga tccgagctcg gtacccaatt cgccctatag

3001 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg

3061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga

3121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt

3181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt

3241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg

3301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc

3361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca

3421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga

3481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa

3541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc

3601 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc

3661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

3721 taacccctga taaatgcttc aataatatga aaaagtaaga

//

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