pSAT6-CEYFP-C1-B_植物细胞载体_质粒载体_U平台商城-普拉特泽生物

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pSAT6-CEYFP-C1-B

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基本信息

产品名称： pSAT6-CEYFP-C1-B
产品编号： P1406
规格： 10μl质粒/300μl菌液
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LOCUS Exported 4081 bp ds-DNA circular SYN 26-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS .

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 4081)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..4081

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 435..1322

/label=2X35S promoter + TL enhancer

misc_feature 1323..1523

/label=nEYFP

misc_feature 1524..1589

/label=MCS

/note="multiple cloning site"

misc_feature 1602..1812

/label=35S terminator

rep_origin complement(2262..2850)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(3021..3881)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(3882..3986)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct

421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag

481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa

541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg

601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct

661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag

721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca

781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga

841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc

901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata

961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac

1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt

1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc

1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa

1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta

1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag

1321 ccatgggcgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg

1381 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc

1441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc

1501 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg

1561 cagtcgacgg taccgcgggc ccgggatcca cctgatctag agtccgcaaa aatcaccagt

1621 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca

1681 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct

1741 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca

1801 aaatccagtg acgcggccgc acccataata cccataatag ctgtttgcca gtaatcatgg

1861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc

1921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg

1981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc

2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact

2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta

2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag

2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc

2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta

2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg

2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc

2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac

2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac

2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg

2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga

2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt

2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag

2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct

2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg

2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat

3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc

3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg

3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct

3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca

3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg

3301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg

3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc

3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag

3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg

3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag

3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat

3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg

3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca

3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca

3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat

3901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag

3961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa

4021 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt

4081 c

//

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