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pSAT6-nEYFP-C1

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基本信息

产品名称： pSAT6-nEYFP-C1
产品编号： P1405
规格： 10μl质粒/300μl菌液
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LOCUS Exported 4402 bp ds-DNA circular SYN 26-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS .

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 4402)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..4402

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 435..1319

/label=2X35S promoter + TL enhancer

CDS 1323..1841

/codon_start=1

/product="N-terminal fragment of mVenus for use in

bimolecular fluorescence complementation (BiFC) (Kodama and

Hu, 2010)"

/label=n(1-174)EYFP

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

VNFKIRHNIE"

misc_feature 1845..1910

/label=MCS

/note="multiple cloning site"

misc_feature 1923..2133

/label=35S terminator

rep_origin complement(2583..3171)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(3342..4202)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(4203..4307)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct

421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag

481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa

541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg

601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct

661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag

721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca

781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga

841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc

901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata

961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac

1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt

1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc

1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa

1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta

1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag

1321 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg

1381 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct

1441 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca

1501 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga

1561 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct

1621 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc

1681 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc

1741 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga

1801 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggactccgga ctcagatctc

1861 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc acctagtcta

1921 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga

1981 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt

2041 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa

2101 aatttctaat tcctaaaacc aaaatccagt gacgcggccg cacccataat acccataata

2161 gctgtttgcc agtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca

2221 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg

2281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg

2341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc

2401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta

2461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag

2521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg

2581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg

2641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg

2701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga

2761 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc

2821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt

2881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact

2941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg

3001 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt

3061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt

3121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct

3181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg

3241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt

3301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt

3361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc

3421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg

3481 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc

3541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg

3601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca

3661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga

3721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct

3781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg

3841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca

3901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata

3961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct

4021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact

4081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa

4141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc

4201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga

4261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga

4321 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg

4381 cgtatcacga ggccctttcg tc

//

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