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pSAT4A-cEYFP-N1

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基本信息

产品名称： pSAT4A-cEYFP-N1
产品编号： P1404
规格： 10μl质粒/300μl菌液
质粒图谱：
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LOCUS Exported 4031 bp ds-DNA circular SYN 26-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS .

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 4031)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..4031

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 413..1158

/label=3×CaMV 35S promoter

misc_feature 1299..1355

/label=MCS

/note="multiple cloning site"

misc_feature 1356..1567

/label=cEYFP

misc_feature 1574..1784

/label=CaMV 35S terminator

rep_origin complement(2212..2800)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(2971..3831)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(3832..3936)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg gtcaacatgt

421 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag

481 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc

541 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca

601 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga

661 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa

721 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat

781 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc

841 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga

901 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga

961 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa

1021 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt

1081 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc

1141 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct

1201 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa

1261 gcaattttct gaaaattttc accatttacg aacgatagag atctcgagct caagcttcga

1321 attctgcagt cgacggtacc gcgggcccgg gatcctgggc agcgtgcagc tcgccgacca

1381 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct

1441 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct

1501 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgaagatc

1561 cacctagtct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat

1621 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt

1681 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact

1741 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacgcggcc gcggcgcgcc

1801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa

1861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac

1921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca

1981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc

2041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc

2101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc

2161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag

2221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc

2281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt

2341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct

2401 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg

2461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct

2521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat

2581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg

2641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa

2701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt

2761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc

2821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt

2881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta

2941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat

3001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac

3061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg

3121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag

3181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt

3241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt

3301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt

3361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt

3421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct

3481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt

3541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac

3601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa

3661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa

3721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca

3781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct

3841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga

3901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc

3961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag

4021 gccctttcgt c

//