您好! 请登录
|
注册
U实验
会员中心
我的订单
我的评价
我的余额
我的红包
我的收藏
修改收货地址
搜索
服务热线:
4006916686
商品分类
哺乳细胞载体
病毒系列载体
大肠杆菌载体
植物细胞载体
酵母系列载体
枯草杆菌载体
昆虫细胞载体
乳酸菌类载体
丝状真菌载体
鱼类系列质粒
杆菌系列质粒
球菌系列质粒
其他宿主载体
RNA文库质粒
其他系统
广宿主质粒
其他
首页
实验官网
细胞系
原代细胞
质粒载体
cDNA克隆
ORF克隆
菌株
首页
>
质粒载体
>
酵母系列载体
>
pDR195
U平台
pDR195
商城价:
询价
¥0元
会员折扣
会员价格
会员等级
会员价格
注册用户
¥0元
vip
¥0元
最近售出
0
件
(
已有 614 人关注
)
购买此商品可使用:
0
积分
数量
-
+
库存(
载入中···
)
加入购物车
在线咨询
收藏
商品简介
购买记录
顾客评价()
基本信息
产品名称:
pDR195
产品编号:
P1398
规格:
10μl质粒/300μl菌液
启动子:
PMA1 promoter
复制子:
pUC ori
终止子:
ADH1 terminator
质粒分类:
酵母细胞质粒;酵母表达质粒;毕赤酵母质粒
质粒大小:
6305bp
原核抗性:
氨苄青霉素Amp
筛选标记:
URA3
克隆菌株:
大肠杆菌DH5α
培养条件:
37℃,有氧,LB
表达宿主:
毕赤酵母
5测序引物:
PMA1-F:CGTTAATAATTAATTAATTGG
3测序引物:
ADH1ter-R:GAGTCACTTTAAAATTTGTATACAC
备注:
高拷贝
质粒简介:
pDR195质粒是一个毕赤酵母表达载体,PMA1启动子驱动目的基因的表达,其参考文献是
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
质粒图谱:
质粒序列:
点击查看序列
LOCUS Exported 6305 bp ds-DNA circular SYN 25-AUG-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6305)
TITLE Direct Submission
JOURNAL Exported Aug 25, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6305
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 510..530
/label=PMA1-F
misc_feature 596..614
/label=MCS
terminator 751..938
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
primer_bind complement(806..830)
/label=ADH1ter-R
rep_origin 1402..2748
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(2859..3662)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(3663..3878)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
promoter 3989..4093
/gene="bla"
/label=AmpR promoter
CDS 4094..4954
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5125..5713
/direction=RIGHT
/label=pUC ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 6127..595
/label=PMA1 promoter
ORIGIN
1 ggagaaactc ctgcgagaat ttcgtaaact cgcgcgcatt gcatctgtat ttcctaatgc
61 ggcacttcca ggcctcgatc gagaccgttt atccattgct tttttgttgt ctttttccct
121 cgttcacaga aagtctgaag aagctatagt agaactatga gctttttttg tttctgtttt
181 cctttttttt ttttttacct ctgtggaaat tgttactctc acactcttta gttcgtttgt
241 ttgttttgtt tattccaatt atgaccggtg acgaaacgtg gtcgatggtg ggtaccgctt
301 atgctcccct ccattagttt cgattatata aaaaggccaa atattgtatt attttcaaat
361 gtcctatcat tatcgtctaa catctaattt ctcttaaatt ttttctcttt ctttcctata
421 acaccaatag tgaaaatctt tttttcttct atatctacaa aaactttttt tttctatcaa
481 cctcgttgat aaattttttc tttaacaatc gttaataatt aattaattgg aaaataacca
541 ttttttctct cttttataca cacattcaaa agaaagaaaa aaaatatacc ccagcctcga
601 gcggccgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
661 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
721 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
781 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
841 aaacgaraat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
901 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca ctggccgtcg
961 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
1021 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
1081 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
1141 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat
1201 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata
1261 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct
1321 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca
1381 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc
1441 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
1501 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
1561 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
1621 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
1681 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
1741 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
1801 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
1861 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
1921 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
1981 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
2041 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
2101 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
2161 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
2221 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
2281 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
2341 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact
2401 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
2461 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
2521 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
2581 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
2641 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
2701 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata
2761 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt
2821 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
2881 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
2941 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
3001 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
3061 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
3121 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
3181 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
3241 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
3301 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
3361 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
3421 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
3481 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
3541 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
3601 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
3661 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg
3721 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
3781 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
3841 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa
3901 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
3961 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4021 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4081 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4141 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4201 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4261 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
4321 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
4381 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
4441 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
4501 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
4561 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
4621 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
4681 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
4741 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
4801 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
4861 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
4921 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
4981 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5041 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5101 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5161 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5281 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5341 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5401 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
&n
▍
购买记录
(近期成交数量
0
)
还没有人购买过此商品
总计 0 个记录,共 1 页。
第一页
上一页
下一页
最末页
▍
用户评论
(共
0
条评论)
暂时还没有任何用户评论
总计 0 个记录,共 1 页。
上一页
下一页
用户名:
匿名用户
E-mail:
评价等级:
评论内容:
验证码:
▍
最近浏览过的商品
购物车(0)
联
系
客
服
U商城
订购指南
订购方式
售后服务
免责协议
售后政策
关于我们
联系我们
关于U商城
联系我们
客服热线:4006916686
在线客服
关注我们