pHBT-GFP-NOS植物绿色荧光表达质粒_植物细胞载体_质粒载体_U平台商城-普拉特泽生物

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pHBT-GFP-NOS植物绿色荧光表达质粒

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基本信息

产品名称： pHBT-GFP-NOS植物绿色荧光表达质粒
产品编号： P1230
规格： 10μl质粒/300μl菌液
启动子： 35s
原核抗性：氨苄青霉素Amp
筛选标记：绿色荧光蛋白EGFP
克隆菌株：大肠杆菌DH5α
培养条件： 37℃
质粒简介： pHBT-GFP-NOS是一个植物细胞绿色荧光表达质粒。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 4281 bp ds-DNA circular SYN 24-MAY-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..4281

/organism="synthetic DNA construct"

/mol_type="other DNA"

primer_bind complement(39..55)

/label=M13 rev

/note="common sequencing primer, one of multiple similar

variants"

protein_bind 63..79

/label=lac operator

/bound_moiety="lac repressor encoded by lacI"

/note="The lac repressor binds to the lac operator to

inhibit transcription in E. coli. This inhibition can be

relieved by adding lactose or

isopropyl-beta-D-thiogalactopyranoside (IPTG)."

promoter complement(87..117)

/label=lac promoter

/note="promoter for the E. coli lac operon"

protein_bind 132..153

/label=CAP binding site

/bound_moiety="E. coli catabolite activator protein"

/note="CAP binding activates transcription in the presence

of cAMP."

rep_origin complement(441..1029)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(1200..2060)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(2061..2165)

/gene="bla"

/label=AmpR promoter

primer_bind 2639..2655

/label=M13 fwd

/note="common sequencing primer, one of multiple similar

variants"

promoter 2722..3231

/label=35SPPDK promoter

/note="hybrid promoter consisting of the cauliflower mosaic

virus 35S enhancer fused to the maize C4PPDK basal promoter

(Yoo et al., 2007)"

misc_feature 3238..3290

/label=MCS

/note="multiple cloning site"

misc_feature 3264..3294

/label=MCS

CDS 3291..4010

/codon_start=1

/product="enhanced GFP"

/label=EGFP

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

EFVTAAGITHGMDELYK"

terminator 4029..4281

/label=NOS terminator

/note="nopaline synthase terminator and poly(A) signal"

ORIGIN

1 cgatgataag ctgtcaaaca tgagaattcg taatcatggt catagctgtt tcctgtgtga

61 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc

121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc

181 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc

241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt

301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca

361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa

421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat

481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc

541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc

601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt

661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac

721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg

781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca

841 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc

901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa

961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa

1021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac

1081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta

1141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt

1201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata

1261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc

1321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac

1381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag

1441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac

1501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc

1561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg

1621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc

1681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct

1741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc

1801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc

1861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc

1921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc

1981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca

2041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt

2101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt

2161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca

2221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac

2281 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat

2341 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg

2401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata

2461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc

2521 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg

2581 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt

2641 aaaacgacgg ccagtgccaa gctctcgaga agcttactcc aagaatatca aagatacagt

2701 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct

2761 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg

2821 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga

2881 cagtggtccc aaagatggac ccccacccac aaggagcatc gtggaaaaag aagacgttcc

2941 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc

3001 acaatcccac tatccttcgc cccaagcttg ggcccaagct tgggtcgcgc cccacggatg

3061 gtataagaat aaaggcattc cgcgtgcagg attcacccgt tcgcctctca ccttttcgct

3121 gtactctctc gccacacaca ccccctctcc agctccgttg gagctccgga cagcagcagg

3181 cgcggggcgg tcacgtagta agcagctctc ggctccctct ccccttgctc cgtggatctc

3241 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc atggtgagca

3301 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa

3361 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga

3421 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca

3481 ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact

3541 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg

3601 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca

3661 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt

3721 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg

3781 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc

3841 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca

3901 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt

3961 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa agcggccgcc

4021 cggctgcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg

4081 gtcttgcgat gattatcata tatatttctg ttgattacgt taagcatgta ataattaaca

4141 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca

4201 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg

4261 tgtcatctat gttactagat c

//

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