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pECMV-3×FLAG-RBM4人源基因哺乳表达质粒
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pECMV-3×FLAG-RBM4人源基因哺乳表达质粒
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基本信息
产品名称:
pECMV-3×FLAG-RBM4人源基因哺乳表达质粒
产品编号:
P1201
规格:
10μl质粒/300μl菌液
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LOCUS Exported 6547 bp ds-DNA circular SYN 12-JUN-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCDNA3.1-3×FLAG-RBM4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6547)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 28, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6547
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 863..881
/label=T7
CDS 922..987
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind 1002..1026
/label=GC87-F
CDS 1009..2103
/codon_start=1
/product="Homo sapiens RNA binding motif protein 4, mRNA
(cDNA clone MGC:45319
IMAGE:5540605), complete cds
GenBank: BC032735.1"
/label=RBM4
/translation="MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDK
TAAEDAIRNLHHYKLHGVNINVEASKNKSKTSTKLHVGNISPTCTNKELRAKFEEYGPV
IECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMHVQLSTSRLRTAPGMGDQSGCY
RCGKEGHWSKECPIDRSGRVADLTEQYNEQYGAVRTPYTMSYGDSLYYNNAYGALDAYY
KRCRAARSYEAVAAAAASVYNYAEQTLSQLPQVQNTAMASHLTSTSLDPYDRHLLPTSG
AAATAAAAAAAAAAVTAASTSYYGRDRSPLRRATAPVPTVGEGYGYGHESELSQASAAA
RNSLYDMARYEREQYADRARYSAF"
primer_bind complement(2084..2109)
/label=GC87-R
primer_bind complement(2141..2158)
/label=BGH
polyA_signal 2147..2371
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2417..2845
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2859..3188
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3039..3174
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3255..4049
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4223..4344
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4393..4409)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4417..4433
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4441..4471)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4486..4507
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4795..5380)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5551..6411)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6412..6516)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccat ggtgaagctg
1021 ttcatcggaa acctgccccg ggaggctaca gagcaggaga ttcgctcact cttcgagcag
1081 tatgggaagg tgctggaatg tgacatcatt aagaattacg gctttgtgca catagaagac
1141 aagacggcag ctgaggatgc catacgcaac ctgcaccatt acaagcttca tggggtgaac
1201 atcaacgtgg aagccagcaa gaataagagc aaaacctcaa caaagttgca tgtgggcaac
1261 atcagtccca cctgcaccaa taaggagctt cgagccaagt ttgaggagta tggtccggtc
1321 atcgaatgtg acatcgtgaa agattatgcc ttcgtacaca tggagcgggc agaggatgca
1381 gtggaggcca tcaggggcct tgataacaca gagtttcaag gcaaacgaat gcacgtgcag
1441 ttgtccacca gccggcttag gactgcgccc gggatgggag accagagcgg ctgctatcgg
1501 tgcgggaaag aggggcactg gtccaaagag tgtccgatag atcgttcagg ccgcgtggca
1561 gacttgaccg agcaatataa tgagcaatac ggagcagtgc gtacgcctta caccatgagc
1621 tatggggatt cattgtatta caacaacgcg tacggagcgc tcgatgccta ctacaagcgc
1681 tgccgtgctg cccggtccta tgaggcagtg gcagctgcag ctgcctccgt gtataattac
1741 gcagagcaga ccctgtccca gctgccacaa gtccagaata cagccatggc cagtcacctc
1801 ac
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