pUC57-Simple-gRNAbackbone爪蟾基因编辑质粒_其他宿主载体_质粒载体_U平台商城-普拉特泽生物

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pUC57-Simple-gRNAbackbone爪蟾基因编辑质粒

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基本信息

产品名称： pUC57-Simple-gRNAbackbone爪蟾基因编辑质粒
产品编号： P1175
规格： 10μl质粒/300μl菌液
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 2845 bp ds-DNA circular SYN 28-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS pUC57-Simple-gRNA backbone

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 2845)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 28, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..2845

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 234..252

/label=T7 promoter

/note="promoter for bacteriophage T7 RNA polymerase"

misc_RNA 271..346

/label=gRNA scaffold

/note="guide RNA scaffold for the Streptococcus pyogenes

CRISPR/Cas9 system"

promoter 888..992

/gene="bla"

/label=AmpR promoter

CDS 993..1853

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 2024..2612

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt

61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt

121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc

181 caagctcgct tgcacgcctc tgcactcgtc ggtcccggca tccgataccc aattaatacg

241 actcactata ggtcgtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat

301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt aaagacccaa

361 tatctcgacg cattcgcgaa gtaccgatct ccaattcact ggccgtcgtt ttacaacgtc

421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg

481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc

541 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac

601 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc

661 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt

721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac

781 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga

841 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta

901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat

961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc

1021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga

1081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca

1141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt

1201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg

1261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc

1321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata

1381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt

1441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag

1501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca

1561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg

1621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg

1681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag

1741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg

1801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag

1861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga

1921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt

1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc

2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc

2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac

2161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac

2221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt

2281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct

2341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat

2401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt

2461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg

2521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt

2581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt

2641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg

2701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg

2761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc

2821 ccgcgcgttg gccgattcat taatg

//

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