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CaV1.3e[8a,11,31b,Δ32,42a]mut大鼠基因哺乳表达质粒
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CaV1.3e[8a,11,31b,Δ32,42a]mut大鼠基因哺乳表达质粒
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基本信息
产品名称:
CaV1.3e[8a,11,31b,Δ32,42a]mut大鼠基因哺乳表达质粒
产品编号:
P1090
规格:
10μl质粒/300μl菌液
启动子:
CMV promoter
复制子:
Ori,F1 ori
终止子:
bGH poly(A) signal
质粒分类:
基因文库质粒;大鼠基因质粒;大鼠真核质粒
质粒大小:
10850bp
原核抗性:
氨苄青霉素Amp
筛选标记:
杀稻瘟素Blast
克隆菌株:
大肠杆菌Top10F
培养条件:
37℃,有氧,LB
表达宿主:
293T等哺乳细胞
质粒简介:
CaV1.3e[8a,11,31b,Δ32,42a] mut质粒的参考文献:
Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines
.
Contains exons 8a, 11, 31b and 42a.Deletion of exon 32. nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
质粒图谱:
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LOCUS Exported 10850 bp ds-DNA circular SYN 17-八月-2017
KEYWORDS CaV1.3e[8a,11,31b,Delta-32,42a] mut
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10850)
FEATURES Location/Qualifiers
source 1..10850
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 901..5832
/note="CaV1.3e[8a,11,31b,Delta-32,42a] mut-r"
/note="Rattus norvegicus voltage-gated calcium channel pore
forming subunit CaV1.3alpha1 mRNA, complete cds,
alternatively spliced
GenBank: AF370009.1"
misc_feature 5833..6679
/note="3'UTR"
primer_bind complement(6663..6679)
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 6812..7036
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 7082..7510
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7524..7853
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 7704..7839
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 7901..7948
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 7967..8365
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/note="Blast"
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 8523..8644
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
protein_bind 8717..8733
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8741..8771)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 8786..8807
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9095..9683)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9854..10714)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(10715..10819)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgcagcatc aacggcagca gcaagaggac cacgcgaacg aggcaaacta tgcaagaggc
961 accagactcc ctatttctgg tgaaggacca acttctcagc cgaacagctc caagcaaacc
1021 gtcctgtctt ggcaagctgc aattgatgct gctagacagg ccaaggccgc ccagaccatg
1081 agcacgtctg cacccccacc tgtaggatct ctctcccaaa gaaaacgtca gcaatacgcc
1141 aagagcaaaa aacagggtaa ctcgtctaac agccgacctg cccgtgccct cttctgttta
1201 tccctcaata accccatccg aagagcctgc attagtatag tggattggaa accatttgac
1261 atatttatat tattggctat ttttgccaat tgtgtggcct tagctattta catcccgttc
1321 cctgaagatg attctaattc aacaaatcat aacttggaaa aagtagaata tgccttcctg
1381 attatcttta cagtggagac atttctgaag attatagcgt acggactgct gctgcatcct
1441 aatgcttacg ttaggaacgg atggaactta ctggattttg ttatagtaat agtaggattg
1501 tttagtgtaa ttttggaaca attaaccaaa gaaacagaag gcgggaacca ctctagtggc
1561 aaatcaggag gctttgatgt caaagccctc cgtgccttcc gagtgttaag accgcttcga
1621 cttgtatcat cagtgcccag tttacaagtt gttctgaact ccattataaa agccatggtc
1681 cccctcctcc acatagccct tttggtgtta ttcgtaatta tcatctatgc gattataggg
1741 ttggaacttt ttattggaaa aatgcacaaa acctgttttt ttgctgactc agatatagta
1801 gctgaagagg acccagctcc atgcgcgttc tcagggaatg gacgccagtg tgccgccaat
1861 ggcacggaat gtaggagcgg ctgggtcggc cccaatggag gcatcactaa ttttgacaac
1921 tttgcctttg ccatgctcac tgtgttccag tgcatcacca tggagggctg gacagatgtg
1981 ctctactggg ttaatgatgc gataggatgg gagtggccat gggtgtattt tgttagtctg
2041 atcatccttg gctcattttt cgtccttaac ctggttcttg gtgtccttag tggagaattc
2101 tcaaaggaaa gagagaaggc taaagcacgt ggagatttcc agaagcttcg ggagaagcag
2161 cagctggaag aggatctcaa gggctacttg gactggatca cccaggcaga agacatagat
2221 cctgagaatg aagaggaggg tggagaggag ggcaaacgaa acactagcat gcccaccagt
2281 gagaccgaat ctgtgaacac agagaacgta agtggtgaag gcgagaccca gggatgctgt
2341 ggaagtctct ggtgctggtg gaaaagaaga ggcgcggcca agactgggcc ctctgggtgt
2401 cggcggtggg gccaagccat ctcgaaatcc aagctcagcc gtcggtggcg tcgctggaac
2461 cggttcaatc gcaggagatg cagggcagct gtgaagtctg tcacgtttta ctggctggtg
2521 atcgttctgg tgtttctcaa cactctgacg atttcctctg agcactacaa tcagccagac
2581 tggctgacgc agattcaaga tatagcgaac aaagtgctct tggctttgtt cacctgtgag
2641 atgctggtga agatgtacag cctgggcctc caggcctact tcgtctctct cttcaaccgc
2701 ttcgattgct ttgtggtgtg tgggggaatc accgaaacca tcctggtgga actggagctc
2761 atgtctcccc tgggcgtctc tgtgttccgg tgtgtacgtc tcctgaggat cttcaaagtg
2821 accaggcact ggacttccct gagcaacttg gtggcgtccc tgttaaactc catgaagtcc
2881 atcgcctctc tgctgttgct gctcttcctc ttcatcatca tcttctccct gctggggatg
2941 cagctgttcg gagggaagtt caatttcgat gagacacaga ccaagcgaag caccttcgac
3001 aacttcccac aggcgctcct gactgtgttc cagatcctga cgggcgaaga ctggaatgcg
3061 gtgatgtatg acggcatcat ggcctacgga ggcccgtcct cctctggaat gattgtctgt
3121 atttacttca tcatcctttt catctgtggt aactatatcc tgctgaacgt cttcttggcc
3181 attgctgtag acaatttggc tgatgctgaa agtctgaaca ctgctcagaa agaggaagcc
3241 gaagaaaaag aaaggaaaaa aattgccaga aaagaaagcc tagaaaacaa aaaaaacaac
3301 aaaccagagg tcaaccagat agccaacagt gacaacaagg ttactattga tgactatcaa
3361 gaggaggctg aagataagga cc
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