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pCAMBIA35S-4×Myc-MCS-3×FLAG植物表达质粒
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pCAMBIA35S-4×Myc-MCS-3×FLAG植物表达质粒
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基本信息
产品名称:
pCAMBIA35S-4×Myc-MCS-3×FLAG植物表达质粒
产品编号:
P1029
规格:
10μl质粒/300μl菌液
启动子:
CaMV 35S promoter
质粒大小:
9847bp
质粒标签:
N-4×Myc,C-3×FLAG
原核抗性:
卡那霉素Kan
筛选标记:
潮霉素Hyg
质粒简介:
pCAMBIA35S-4×Myc-MCS-3×FLAG质粒是一个植物系列质粒。
质粒图谱:
质粒序列:
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LOCUS Exported 9847 bp ds-DNA circular SYN 11-OCT-2017
DEFINITION synthetic circular DNA
KEYWORDS pCAMBIA35S-4×Myc-MCS-3×FLAG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9847)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..9847
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 234..579
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 599..718
/codon_start=1
/product="3 tandem Myc epitope tags"
/label=4×Myc
/translation="EQKLISEEDLEQKLISEEDLEQKLISEEDLEQKLISEEDL"
misc_feature 719..802
/label=MCS
/note="pUC18/19 multiple cloning site"
CDS 803..868
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 894..1146
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 1168..1192
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 2492..3121
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 3555..4622
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 4688..4882
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 5226..5366
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(5552..6140)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6227..7021)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=Kan
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 7446..7470
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 7548..7722
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(7762..8787)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=Hyg
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(8855..9532)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 9723..9744
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9759..9789
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9797..9813
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
1 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttagctt catggagtca
61 aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag
121 agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca
181 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
241 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
301 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
361 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
421 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
481 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
541 tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct tgaccatgga
601 acaaaaacta atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga
661 gcaaaagtta atttccgagg aggacttaga gcagaagttg atttcagaag aagatctggg
721 gcccataggc ctgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
781 tgcaagctta ctagtcacgc gtgactacaa agaccatgac ggtgattata aagatcatga
841 catcgattac aaggatgacg atgacaagta aggtgaccag ctcgaatttc cccgatcgtt
901 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
961 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
1021 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
1081 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
1141 tagatcggga attaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
1201 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt
1261 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
1321 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
1381 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
1441 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
1501 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
1561 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
1621 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
1681 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
1741 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
1801 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
1861 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
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