pHCMC04枯草整合木糖诱导表达质粒_枯草杆菌载体_质粒载体_U平台商城-普拉特泽生物

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pHCMC04枯草整合木糖诱导表达质粒

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基本信息

产品名称： pHCMC04枯草整合木糖诱导表达质粒
产品编号： P1811
规格： 10μl质粒/300μl菌液
启动子： xylA promoter
原核抗性：氨苄青霉素Amp
筛选标记：氯霉素Chl
克隆菌株：大肠杆菌DH5α
培养条件： 37℃
表达宿主：枯草芽孢杆菌
诱导方式：木糖
质粒简介： pHCMC04是一个枯草芽孢杆菌整合木糖诱导表达质粒。
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LOCUS Exported 8089 bp ds-DNA circular SYN 06-JAN-2018

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..8089

/organism="synthetic DNA construct"

/mol_type="other DNA"

rep_origin complement(440..1028)

/direction=LEFT

/label=ori

/note="high-copy-number colE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(1199..2059)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(2060..2164)

/gene="bla"

/label=AmpR promoter

CDS complement(2343..2993)

/codon_start=1

/label=Chl

/translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE

GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV

KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL

LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"

CDS complement(3497..4621)

/codon_start=1

/label=xylR

/translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI

FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS

ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL

IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG

FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV

EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD

NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"

misc_feature 4729..4882

/label=xylA promoter

misc_feature 4805..4829

/label=xylO

misc_feature 4898..4997

/label=MCS

ORIGIN

1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa

61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct

121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc

181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg

241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc

301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag

361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa

421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc

481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc

541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg

601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt

661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc

721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc

781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag

841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg

901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa

961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag

1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact

1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa

1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt

1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag

1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca

1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc

1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt

1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg

1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca

1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg

1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca

1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg

1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct

1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca

1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca

1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg

1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac

2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt

2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc

2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg

2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg

2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca

2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat

2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt

2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta

2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa

2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca

2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca

2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa

2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt

2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat

2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga

2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat

3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta

3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat

3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac

3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc

3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc

3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga

3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc

3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc

3481 cagtgaattc gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag

3541 aaaccgctcc taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat

3601 tgtctaaatg agaatggact ctagaagaaa cttcgttttt aatcgtattt aaaacaatgg

3661 gatgagattc aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga

3721 tattggttaa tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta

3781 catcattttt atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag

3841 agagcgaagc cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac

3901 agctgcattt gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct

3961 gaacaccttt atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac

4021 tgatgtaaac gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc

4081 ctgcattagc ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact

4141 gcaaatcttt gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta

4201 caaggcctgg aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg

4261 ttacaaaacc atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag

4321 acaagtcaga agtcttctcg agaataatat ttccttctaa gtcggttaga attccgttaa

4381 gatagtcgac tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca

4441 ttacaggtct tctgccgcct ctagattgcc ctgccccaat ttcaaaaata aaatcttttt

4501 caagcagtgt atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga

4561 gagttgccct ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca

4621 ttttttttac taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc

4681 gaactaagtt ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat

4741 tataactaaa ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat

4801 aaagttagtt tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt

4861 tcacttaaat caaaggggga aatgacaaat ggtccaaact agtgatatct aaaaatcaaa

4921 gggggaaatg ggatcctcta gagtcgacgt ccccggggca gcccgcctaa tgagcgggct

4981 tttttcacgt cacgcgtcca tggagatctt tgtctgcaac tgaaaagttt ataccttacc

5041 tggaacaaat ggttgaaaca tacgaggcta atatcggctt attaggaata gtccctgtac

5101 taataaaatc aggtggatca gttgatcagt atattttgga cgaagctcgg aaagaatttg

5161 gagatgactt gcttaattcc acaattaaat taagggaaag aataaagcga tttgatgttc

5221 aaggaatcac ggaagaagat actcatgata aagaagctct aaaactattc aataacctta

5281 caatggaatt gatcgaaagg gtggaaggtt aatggtacga aaattagggg atctacctag

5341 aaagccacaa ggcgataggt caagcttaaa gaacccttac atggatctta cagattctga

5401 aagtaaagaa acaacagagg ttaaacaaac agaaccaaaa agaaaaaaag cattgttgaa

5461 aacaatgaaa gttgatgttt caatccataa taagattaaa tcgctgcacg aaattctggc

5521 agcatccgaa gggaattcat attacttaga ggatactatt gagagagcta ttgataagat

5581 ggttgagaca ttacctgaga gccaaaaaac tttttatgaa tatgaattaa aaaaaagaac

5641 caacaaaggc tgagacagac tccaaacgag tctgtttttt taaaaaaaat attaggagca

5701 ttgaatatat attagagaat taagaaagac atgggaataa aaatatttta aatccagtaa

5761 aaatatgata agattatttc agaatatgaa gaactctgtt tgtttttgat gaaaaaacaa

5821 acaaaaaaaa tccacctaac ggaatctcaa tttaactaac agcggccaaa ctgagaagtt

5881 aaatttgaga aggggaaaag gcggatttat acttgtattt aactatctcc attttaacat

5941 tttattaaac cccatacaag tgaaaatcct cttttacact gttcctttag gtgatcgcgg

6001 agggacatta tgagtgaagt aaacctaaaa ggaaatacag atgaattagt gtattatcga

6061 cagcaaacca ctggaaataa aatcgccagg aagagaatca aaaaagggaa agaagaagtt

6121 tattatgttg ctgaaacgga agagaagata tggacagaag agcaaataaa aaacttttct

6181 ttagacaaat ttggtacgca tataccttac atagaaggtc attatacaat cttaaataat

6241 tacttctttg atttttgggg ctatttttta ggtgctgaag gaattgcgct ctatgctcac

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