pmCherry-LC3a哺乳细胞人源自噬质粒_其他_质粒载体_U平台商城-普拉特泽生物

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pmCherry-LC3a哺乳细胞人源自噬质粒

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基本信息

产品名称： pmCherry-LC3a哺乳细胞人源自噬质粒
产品编号： P1746
规格： 10μl质粒/300μl菌液
启动子： CMV
原核抗性：卡那霉素Kan
筛选标记：遗传霉素G418
克隆菌株：大肠杆菌DH5α
培养条件： 37℃
质粒简介： pmCherry-LC3a是一个哺乳细胞人源基因自噬表达质粒。
质粒图谱：

质粒序列：点击查看序列

LOCUS Exported 5080 bp ds-DNA circular SYN 20-DEC-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..5080

/organism="synthetic DNA construct"

/mol_type="other DNA"

enhancer 61..364

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 365..568

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

regulatory 607..616

/regulatory_class="other"

/note="vertebrate consensus sequence for strong initiation

of translation (Kozak, 1987)"

CDS 613..1320

/codon_start=1

/product="monomeric derivative of DsRed fluorescent protein

(Shaner et al., 2004)"

/label=mCherry

/note="mammalian codon-optimized"

/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG

TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF

EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK

GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA

EGRHSTGGMDELYK"

CDS 1342..1704

/codon_start=1

/gene="MAP1LC3A"

/product="human LC3 protein"

/label=LC3a

/note="Atg8 family member found on autophagosomes"

/translation="MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLP

VLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKD

EDGFLYMVYASQETFGF"

polyA_signal 1868..1989

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin complement(1996..2451)

/direction=LEFT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 2478..2582

/gene="bla"

/label=AmpR promoter

promoter 2584..2941

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 2792..2927

/label=SV40 ori

/note="SV40 origin of replication"

CDS 2976..3770

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/label=NeoR/KanR

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

polyA_signal 4002..4049

/label=HSV TK poly(A) signal

/note="herpes simplex virus thymidine kinase

polyadenylation signal (Cole and Stacy, 1985)"

rep_origin 4378..4966

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc

661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc

721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt

781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc

841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc

901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc

961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc

1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac

1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc

1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc

1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg

1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag

1321 tccggactca gatctcgagc tatgccctca gaccggcctt tcaagcagcg gcggagcttc

1381 gccgaccgct gtaaggaggt acagcagatc cgcgaccagc accccagcaa aatcccggtg

1441 atcatcgagc gctacaaggg tgagaagcag ctgcccgtcc tggacaagac caagtttttg

1501 gtcccggacc atgtcaacat gagcgagttg gtcaagatca tccggcgccg cctgcagctg

1561 aaccccacgc aggccttctt cctgctggtg aaccagcaca gcatggtgag tgtgtccacg

1621 cccatcgcgg acatctacga gcaggagaaa gacgaggacg gcttcctcta tatggtctac

1681 gcctcccagg aaaccttcgg cttctgagaa ttctgcagtc gacggtaccg cgggcccggg

1741 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact

1801 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt

1861 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa

1921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa

1981 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt

2041 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa

2101 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa

2161 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac

2221 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga

2281 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa

2341 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc

2401 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca

2461 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata

2521 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga

2581 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc

2641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg

2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc

2761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc

2821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc

2881 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa

2941 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg

3001 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa

3061 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg

3121 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt

3181 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa

3241 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc

3301 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg

3361 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg

3421 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg

3481 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg

3541 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact

3601 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg

3661 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc

3721 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct

3781 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac

3841 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat

3901 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga

3961 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa

4021 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca

4081 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc

4141 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc

4201 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt

4261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta

4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg

4381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc

4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag

4501 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa

4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc

4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc

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