pDC316-mCMV-EGFP腺病毒Admax系列表达质粒_病毒系列载体_质粒载体_U平台商城-普拉特泽生物

首页 > 质粒载体 > 病毒系列载体 > pDC316-mCMV-EGFP腺病毒Admax系列表达质粒

U平台

pDC316-mCMV-EGFP腺病毒Admax系列表达质粒

商城价：询价￥0元会员折扣

会员价格

会员等级	会员价格
注册用户	￥0元
vip	￥0元

最近售出0件（已有 1693 人关注）
购买此商品可使用：0积分

数量

- + 库存(载入中···)

加入购物车

在线咨询收藏

商品简介
购买记录
顾客评价()

基本信息

产品名称： pDC316-mCMV-EGFP腺病毒Admax系列表达质粒
产品编号： P1691
规格： 10μl质粒/300μl菌液
原核抗性：氨苄青霉素Amp
筛选标记：绿色荧光蛋白EGFP
克隆菌株：大肠杆菌Stbl3
培养条件： 37℃
质粒简介： pDC316-mCMV-EGFP是一个腺病毒Admax系列表达质粒。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 5879 bp ds-DNA circular SYN 10-DEC-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..5879

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 468..992

/label=mCMV promoter

CDS 999..1715

/codon_start=1

/product="enhanced GFP"

/label=EGFP

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

EFVTAAGITLGMDELYK"

polyA_signal 1862..1983

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

enhancer 1991..2370

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 2371..2574

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

promoter 2619..2637

/label=T7 promoter

/note="promoter for bacteriophage T7 RNA polymerase"

misc_feature 2651..2740

/label=MCS

polyA_signal 2764..2988

/label=bGH poly(A) signal

/note="bovine growth hormone polyadenylation signal"

polyA_signal 3002..3136

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

protein_bind 3156..3189

/label=loxP

/bound_moiety="Cre recombinase"

/note="Cre-mediated recombination occurs in the 8-bp core

sequence (GCATACAT)."

rep_origin complement(3613..4201)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(4372..5232)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(5233..5337)

/gene="bla"

/label=AmpR promoter

misc_feature 5420..453

/label=Ad

ORIGIN

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt

61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg

121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg

181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt

241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa

301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg

361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc

421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga

481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc

541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa

601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat

661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc

721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat

781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg

841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct

901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca

961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcat ggtgagcaag ggcgaggagc

1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt

1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca

1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg

1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg

1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca

1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg

1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca

1441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga

1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc

1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc

1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg

1681 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagatccac

1741 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt

1801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt

1861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac

1921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc

1981 ttaacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta

2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc

2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg

2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg

2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa

2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac

2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg

2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg

2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca

2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg

2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag

2641 acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc actgtgctgg

2701 atatctgcag aattgatccg agctcggtac caagcttaag tttaaaccgc tgatcagcct

2761 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga

2821 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt

2881 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg

2941 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt cgacttcgag

3001 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac

3061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc

3121 ttatcatgtc tggatcgtct agcatcgaag atccaataac ttcgtatagc atacattata

3181 cgaagttata agtagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta

3241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc

3301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg

3361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg

3421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg

3481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa

3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc

3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc

3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag

3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct

3781 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta

3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc

3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc

3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt

4021 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct

4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc

4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca

4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta

4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa

4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg

4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg

4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc

4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc

4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa

4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc

4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg

4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc

4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat

4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg

4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc

4981 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg

5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat

5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg

5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg

5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct