pUC35s-mCherry植物红色荧光瞬转表达质粒_植物细胞载体_质粒载体_U平台商城-普拉特泽生物

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pUC35s-mCherry植物红色荧光瞬转表达质粒

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基本信息

产品名称： pUC35s-mCherry植物红色荧光瞬转表达质粒
产品编号： P1677
规格： 10μl质粒/300μl菌液
原核抗性：氨苄青霉素
克隆菌株：大肠杆菌DH5α
质粒简介： pUC35s-mCherry植物红色荧光瞬转表达质粒，
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 4053 bp ds-DNA circular SYN 01-DEC-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..4053

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 439..784

/label=CaMV 35S promoter

/note="strong constitutive promoter from cauliflower mosaic

virus"

CDS 823..1533

/codon_start=1

/product="monomeric derivative of DsRed fluorescent protein

(Shaner et al., 2004)"

/label=mCherry

/note="mammalian codon-optimized"

/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG

TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF

EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK

GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA

EGRHSTGGMDELYK"

terminator 1550..1802

/label=NOS terminator

/note="nopaline synthase terminator and poly(A) signal"

rep_origin complement(2234..2822)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(2993..3853)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(3854..3958)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcaaag atacagtctc

421 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg

481 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc

541 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag

601 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac

661 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca

721 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag

781 aacacggggg actctagagg atccccgggt ggtcagtccc ttatggtgag caagggcgag

841 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc

901 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc

961 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg

1021 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac

1081 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac

1141 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag

1201 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg

1261 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc

1321 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac

1381 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc

1441 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac

1501 tccaccggcg gcatggacga gctgtacaag tgagagctcg aatttccccg atcgttcaaa

1561 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat

1621 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt

1681 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa

1741 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga

1801 tcggggtacc gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt

1861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg

1921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg

1981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc

2041 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc

2101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata

2161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg

2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct

2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa

2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc

2401 tcccttcggg aagcgtggcg ctttctcaaa gctcacgctg taggtatctc agttcggtgt

2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg

2521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg

2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct

2641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc

2701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg

2761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc

2821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt

2881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa

2941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat

3001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct

3061 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg

3121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag

3181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta

3241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg

3301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg

3361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct

3421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta

3481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg

3541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc

3601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg

3661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga

3721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg

3781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat

3841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc

3901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca

3961 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct

4021 ataaaaatag gcgtatcacg aggccctttc gtc

//

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