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SaBE4-Gam哺乳基因编辑质粒
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SaBE4-Gam哺乳基因编辑质粒
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基本信息
产品名称:
SaBE4-Gam哺乳基因编辑质粒
产品编号:
P1673
规格:
10μl质粒/300μl菌液
原核抗性:
氨苄青霉素Amp
克隆菌株:
大肠杆菌Stbl3
培养条件:
37℃
质粒简介:
SaBE4-Gam是一个大鼠基因哺乳细胞过表达质粒。
质粒图谱:
质粒序列:
点击查看序列
LOCUS Exported 8601 bp ds-DNA circular SYN 01-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8601
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 403..412
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 409..930
/label=Gam
CDS 979..1662
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
misc_feature 1759..4917
/label=SaCas9
CDS 4924..4944
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4951..5016
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 5047..5295
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5326..5574
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5587..5607
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5616..5633
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 5662..5886
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(6359..6947)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7118..7978)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7979..8083)
/gene="bla"
/label=AmpR promoter
enhancer 8353..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctaaacca
421 gcaaaacgta tcaagagtgc cgcagcggct tatgtgccac aaaaccgcga tgcggtgatt
481 accgatatta aacgcatcgg ggatttacag cgcgaagcat cacgtctgga aacggaaatg
541 aatgatgcca tcgcggaaat tacggagaaa tttgcggccc ggattgcacc gattaaaacc
601 gatattgaaa ccctttcaaa aggcgttcag ggatggtgtg aagcgaaccg cgacgaactg
661 acgaacggcg gcaaagtgaa gacggcgaat cttgtcaccg gtgatgtatc gtggcgggtc
721 cgtccaccat cagtaagtat tcgtggtatg gatgcagtga tggaaacgct ggagcgtctt
781 ggcctgcaac gctttattcg cacgaagcag gaaatcaaca aggaagcgat tttactggaa
841 ccgaaagcgg tcgcaggcgt tgccggaatt acagttaaat caggcattga ggatttttct
901 attattccat ttgaacagga agccggtatt agcggcagcg agactcccgg gacctcagag
961 tccgctacac ccgaaagtag ctcagagact ggcccagtgg ctgtggaccc cacattgaga
1021 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag
1081 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca
1141 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat
1201 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa
1261 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac
1321 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc
1381 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac
1441 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta
1501 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt
1561 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac
1621 cagcgactgc ccccacacat tctctgggcc accgggttga aatctggtgg ttcttctggt
1681 ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagttctggt
1741 ggttcttctg gtggttctgg gaaacggaac tacatcctgg ggcttgccat tgggataacc
1801 agcgttggct acggaattat tgattatgag acacgcgatg tgattgacgc cggggttagg
1861 ctgttcaaag aggccaacgt tgaaaacaac gagggaagac ggagtaagcg cggagcaaga
1921 agactcaagc gcagacggag acatcggatt cagagggtga aaaagctgct cttcgattac
1981 aatctcctga ccgatcatag tgagctgagc ggaatcaacc cctacgaggc gcgagtgaaa
2041 gggctttccc agaagctgtc cgaagaggag ttctccgccg cgttgctgca cctggccaaa
2101 cggagggggg ttcacaatgt aaacgaagtg gaggaggaca cgggcaatga acttagtacg
2161 aaagaacaga tcagtaggaa ctctaaggct ctcgaagaga aatacgtcgc tgagttgcag
2221 cttgagagac tgaaaaaaga cggcgaagta cgcggatcta ttaataggtt caagacttca
2281 gattacgtaa aggaagccaa gcagctcctg aaagtacaga aagcgtacca tcagctcgat
2341 cagagcttca tcgataccta catagatttg ctggagacac ggaggacata ctacgagggc
2401 ccaggggaag gatctccttt tgggtggaag gacatcaagg aatggtacga gatgcttatg
2461 ggacattgta catattttcc ggaggagctc aggagcgtca agtacgccta caatgccgac
2521 ctgtacaatg ccctcaatga cctcaataac ctcgtgatta ccagggacga gaacgagaag
2581 ctggagtact atgaaaagtt ccagattatc gagaatgtgt ttaagcagaa gaagaagccg
2641 acacttaagc agattgcaaa ggaaatcctc gtgaatgagg aagatatcaa gggatacaga
2701 gtgacaagta caggcaagcc cgagttcaca aatctgaagg tgtaccacga tattaaggac
2761 ataaccgcac gaaaggagat aatcgaaaac gctgagctcc tcgatcagat cgcaaaaatt
2821 cttaccatct accagtctag tgaggacatt caggaggaac tgactaatct gaacagtgag
2881 ctcacccaag aggaaattga gcagatttca aacctgaaag gctacaccgg gacgcacaat
2941 ctgagcctca aagcaatcaa cctcattctg gatgaacttt ggcacacaaa tgacaaccaa
3001 attgccatat tcaaccgcct gaaactggtg ccaaaaaaag tggatctgtc acagcaaaag
3061 gaaatcccta caaccttggt tgacgatttt attctgtccc ccgttgtcaa gcggagcttc
3121 atccagtcaa tcaaggtgat caatgccatc attaaaaaat acggattgcc aaacgatata
3181 attatcgagc ttgcacgaga gaagaactca aaggacgccc agaagatgat taacgaaatg
3241 cagaagcgca accgccagac aaacgaacgc atagaggaaa ttataagaac aaccggcaaa
3301 gagaatgcca agtatctgat cgagaaaatc aagctgcacg acatgcaaga aggcaagtgc
&nb
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