pT040酵母gRNA表达质粒_酵母系列载体_质粒载体_U平台商城-普拉特泽生物

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pT040酵母gRNA表达质粒

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基本信息

产品名称： pT040酵母gRNA表达质粒
产品编号： P1660
规格： 10μl质粒/300μl菌液
原核抗性：氨苄青霉素Amp
筛选标记： URA3
克隆菌株：大肠杆菌Stbl3
培养条件： 37℃
质粒简介： pT040是一个酵母细胞gRNA表达质粒。
质粒图谱：
质粒序列：点击查看序列

LOCUS Exported 5186 bp ds-DNA circular SYN 25-NOV-2017

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..5186

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 27..131

/gene="bla"

/label=AmpR promoter

CDS 132..992

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 1163..1751

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

terminator 2378..2397

/label=SUP4 terminator

/note="transcription terminator for the S. cerevisiae SUP4

tRNA gene"

promoter complement(2479..2747)

/label=SNR52 promoter

/note="promoter for the S. cerevisiae small nucleolar RNA

gene SNR52"

promoter 4089..4304

/gene="S. cerevisiae URA3"

/label=URA3 promoter

CDS 4305..5108

/codon_start=1

/gene="S. cerevisiae URA3"

/product="orotidine-5'-phosphate decarboxylase, required

for uracil biosynthesis"

/label=URA3

/note="yeast auxotrophic marker, counterselectable with

5-fluoroorotic acid (5-FOA)"

/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL

ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ

YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE

YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD

VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

ORIGIN

1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac

61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa

121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat

181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc

241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga

301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg

361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc

421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag

481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc

541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg

601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg

661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac

721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac

781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg

841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg

901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg

961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac

1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg

1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg

1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc

1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc

1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt

1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc

1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact

1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac

1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag

1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg

1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg

1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga

1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt

1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct

1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg

1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt

1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta

2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta

2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt

2161 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct

2221 cgatcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc ccgctcgagc

2281 gggggggatc cactagttct agagcggccg tggtatcgtt tagattggca attacagtgt

2341 cttagctcac atgcttataa ctaattacat gactcgaaga cataaaaaac aaaaaaagca

2401 ccaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta

2461 tttaaatgca tcgattctga tcatttatct ttcactgcgg agaagtttcg aacgccgaaa

2521 catgcgcacc aactttcact tctacagcgt ttgaccaaaa tcttttgaac agaacattgt

2581 agggtgtgaa aaaatgcgca cctttaccgc tagcccaaga gggcactaca aaatctagag

2641 ttgtacttca aacgtacatg taatcacctt gtatatactc gaaagaaaac atcaagtttc

2701 tgtataaata tgagtgaaag cataatcata cattatcttt tcaaagattg tccgcggtgg

2761 agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt

2821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc

2881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc

2941 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca

3001 caccgcataa ctagctttta acttgtatcc taggttatct atgctgtctc accatagaga

3061 atattaccta tttcagaatg tatgtccatg attcgccggg taaatacata taatacacaa

3121 atctggctta ataaagtcta taatatatct cataaagaag tgctaaattg gctagtgcta

3181 tatattttta agaaaatttc ttttgactaa gtccatatcg actttgtaaa agttcacttt

3241 agcatacata tattacacga gccagaaatt gtaacttttg cctaaaatca caaattgcaa

3301 aatttaattg cttgcaaaag gtcacatgct tataatcaac ttttttaaaa atttaaaata

3361 cttttttatt ttttattttt aaacataaat gaaataattt atttattgtt tatgattacc

3421 gaaacataaa acctgctcaa gaaaaagaaa ctgttttgtc cttggaaaaa aagcactacc

3481 taggagcggc caaaatgccg aggctttcat agcttaaact ctttacagaa aataggcatt

3541 atagatcagt tcgagttttc ttattcttcc ttccggtttt atcgtcacag ttttacagta

3601 aataagtatc acctcttaga gttggcggtt gtttgcaaga ccgagaaaag gctagcaaga

3661 atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt gattccattg

3721 cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa tcaatcaaaa

3781 agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta tgtttagatc

3841 ttttatgctt gcttttcaaa aggcctgcag gcaagtgcac aaacaatact taaataaata

3901 ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc

3961 ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct

4021 aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctaag

4081 cttttcaatt caattcatca tttttttttt attctttttt ttgatttcgg tttctttgaa

4141 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta

4201 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa

4261 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat

4321 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc

4381 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg

4441 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga

4501 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt

4561 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact

4621 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg

4681 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag

4741 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta

4801 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa

4861 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt

4921 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta

4981 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg

5041 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc

5101 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt

5161 caatttaatt atatcagtta ttaccc

//

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