您好! 请登录
|
注册
U实验
会员中心
我的订单
我的评价
我的余额
我的红包
我的收藏
修改收货地址
搜索
服务热线:
4006916686
商品分类
哺乳细胞载体
病毒系列载体
大肠杆菌载体
植物细胞载体
酵母系列载体
枯草杆菌载体
昆虫细胞载体
乳酸菌类载体
丝状真菌载体
鱼类系列质粒
杆菌系列质粒
球菌系列质粒
其他宿主载体
RNA文库质粒
其他系统
广宿主质粒
其他
首页
实验官网
细胞系
原代细胞
质粒载体
cDNA克隆
ORF克隆
菌株
首页
>
质粒载体
>
哺乳细胞载体
>
MSP680
U平台
MSP680
商城价:
询价
¥0元
会员折扣
会员价格
会员等级
会员价格
注册用户
¥0元
vip
¥0元
最近售出
0
件
(
已有 528 人关注
)
购买此商品可使用:
0
积分
数量
-
+
库存(
载入中···
)
加入购物车
在线咨询
收藏
商品简介
购买记录
顾客评价()
基本信息
产品名称:
MSP680
产品编号:
P1651
规格:
10μl质粒/300μl菌液
原核抗性:
氨苄青霉素Amp
克隆菌株:
大肠杆菌Stbl3
培养条件:
37℃
质粒简介:
MSP680是一个哺乳CAS9表达质粒。
质粒图谱:
质粒序列:
点击查看序列
LOCUS Exported 7614 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..7614
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 403..412
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 409..4512
/codon_start=1
/product="Streptococcus pyogenes Cas9 endonuclease with the
D1135V/R1335Q/T1337R mutations"
/label=Cas9 VQR
/note="has an altered recognition specificity for the
protospacer adjacent motif (PAM) (Kleinstiver et al.,
2015)"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFESPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 4519..4539
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4546..4611
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 4675..4899
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(5372..5960)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6131..6991)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6992..7096)
/gene="bla"
/label=AmpR promoter
enhancer 7366..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggataaaaag
421 tattctattg gtttagacat cggcactaat tccgttggat gggctgtcat aaccgatgaa
481 tacaaagtac cttcaaagaa atttaaggtg ttggggaaca cagaccgtca ttcgattaaa
541 aagaatctta tcggtgccct cctattcgat agtggcgaaa cggcagaggc gactcgcctg
601 aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc gaatatgtta cttacaagaa
661 atttttagca atgagatggc caaagttgac gattctttct ttcaccgttt ggaagagtcc
721 ttccttgtcg aagaggacaa gaaacatgaa cggcacccca tctttggaaa catagtagat
781 gaggtggcat atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa gctagttgac
841 tcaactgata aagcggacct gaggttaatc tacttggctc ttgcccatat gataaagttc
901 cgtgggcact ttctcattga gggtgatcta aatccggaca actcggatgt cgacaaactg
961 ttcatccagt tagtacaaac ctataatcag ttgtttgaag agaaccctat aaatgcaagt
1021 ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta aatcccgacg gctagaaaac
1081 ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc
1141 tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg
1201 cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat
1261 cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac
1321 atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg
1381 tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct
1441 gagaaatata aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac
1501 ggcggagcga gtcaagagga attctacaag tttatcaaac ccatattaga gaagatggat
1561 gggacggaag agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa gcagcggact
1621 ttcgacaacg gtagcattcc acatcaaatc cacttaggcg aattgcatgc tatacttaga
1681 aggcaggagg atttttatcc gttcctcaaa gacaatcgtg aaaagattga gaaaatccta
1741 acctttcgca taccttacta tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg
1801 atgacaagaa agtccgaaga aacgattact ccatggaatt ttgaggaagt tgtcgataaa
1861 ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact ttgacaagaa tttaccgaac
1921 gaaaaagtat tgcctaagca cagtttactt tacgagtatt tcacagtgta caatgaactc
1981 acgaaagtta agtatgtcac tgagggcatg cgtaaacccg cctttctaag cggagaacag
2041 aagaaagcaa tagtagatct gttattcaag accaaccgca aagtgacagt taagcaattg
2101 aaagaggact actttaagaa aattgaatgc ttcgattctg tcgagatctc cggggtagaa
2161 gatcgattta atgcgtcact tggtacgtat catgacctcc taaagataat taaagataag
2221 gacttcctgg ataacgaaga gaatgaagat atcttagaag atatagtgtt gactcttacc
2281 ctctttgaag atcgggaaat gattgaggaa agactaaaaa catacgctca cctgttcgac
2341 gataaggtta tgaaacagtt aaagaggcgt cgctatacgg gctggggacg attgtcgcgg
2401 aaacttatca acgggataag agacaagcaa agtggtaaaa ctattctcga ttttctaaag
2461 agcgacggct tcgccaatag gaactttatg cagctgatcc atgatgactc tttaaccttc
2521 aaagaggata tacaaaaggc acaggtttcc ggacaagggg actcattgca cgaacatatt
2581 gcgaatcttg ctggttcgcc agccatcaaa aagggcatac tccagacagt caaagtagtg
2641 gatgagctag ttaaggtcat gggacgtcac aaaccggaaa acattgtaat cgagatggca
2701 cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc gagagcggat gaagagaata
2761 gaagagggta ttaaagaact gggcagccag atcttaaagg agcatcctgt ggaaaatacc
2821 caattgcaga acgagaaact ttacctctat tacctacaaa atggaaggga catgtatgtt
2881 gatcaggaac tggacataaa ccgtttatct gattacgacg tcgatcacat tgtaccccaa
2941 tcctttttga aggacgattc aatcgacaat aaagtgctta cacgctcgga taagaaccga
3001 gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg
3061 cagctcctaa atgcgaaact gataacgcaa agaaagttcg ataacttaac taaagctgag
3121 aggggtggct tgtctgaact tgacaaggcc ggatttatta aacgtcagct cgtggaaacc
3181 cgccaaatca caaagcatgt tgcacagata ctagattccc gaatgaatac gaaatacgac
3241 gagaacgata agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg
3301 gacttcagaa aggattttca attctataaa gttagggaga taaataacta ccaccatgcg
3361 cacgacgctt atcttaatgc cgtcgtaggg accgcactca ttaagaaata cccgaagcta
3421 gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa
3481 agcgaacagg agataggcaa ggctacagcc aaatacttct tttattctaa cattatgaat
3541 ttctttaaga cggaaatcac tctggcaaac ggagagatac gcaaacgacc tttaattgaa
3601 accaatgggg agacaggtga aatcgtatgg gataagggcc gggacttcgc gacggtgaga
▍
购买记录
(近期成交数量
0
)
还没有人购买过此商品
总计 0 个记录,共 1 页。
第一页
上一页
下一页
最末页
▍
用户评论
(共
0
条评论)
暂时还没有任何用户评论
总计 0 个记录,共 1 页。
上一页
下一页
用户名:
匿名用户
E-mail:
评价等级:
评论内容:
验证码:
▍
最近浏览过的商品
人C1orf158(NM_152290)ORF克隆
¥0元
¥0元
大鼠 Cd96 (NM_001025032) cDNA克隆
¥0元
¥0元
[清空]
购物车(0)
联
系
客
服
U商城
订购指南
订购方式
售后服务
免责协议
售后政策
关于我们
联系我们
关于U商城
联系我们
客服热线:4006916686
在线客服
关注我们