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pCXUN
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pCXUN
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基本信息
产品名称:
pCXUN
产品编号:
P1620
规格:
10μl质粒/300μl菌液
启动子:
Ubi promoter
终止子:
NOS terminator
质粒大小:
12008bp
原核抗性:
卡那霉素Kan
筛选标记:
潮霉素Hyg
克隆菌株:
大肠杆菌DB3.1
培养条件:
37℃,有氧,LB
质粒简介:
pCXUN质粒是一个植物过表达载体,Ubi启动子驱动目的基因的表达。使用XcmI酶切空载体得到T载体,可连入两端带A的PCR产物,并在ccdb致死基因的帮助下,基因克隆的阳性率得以大大提高。
质粒图谱:
质粒序列:
点击查看序列
LOCUS Exported 12008 bp ds-DNA circular SYN 30-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12008)
AUTHORS .
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..12008
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 115..367
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(439..744)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
promoter complement(1101..3091)
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
misc_feature 3332..3356
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4655..5284
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5718..6785
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 6851..7045
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 7389..7529
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7715..8303)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8390..9184)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 9609..9633
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 9711..9885
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(9925..10950)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(11017..11694)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
ORIGIN
1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
361 aacgatcggg gaaattcgga tccccaatac ttgtatgggt cgacctgcag actggctgtg
421 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
481 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
541 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
601 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
661 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
721 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
781 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
841 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
901 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
961 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
1021 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccccataca
1081 agtattgggg atcccccggg ctgcagaagt aacaccaaac aacagggtga gcatcgacaa
1141 aagaaacagt accaagcaaa taaatagcgt atgaaggcag ggctaaaaaa atccacatat
1201 agctgctgca tatgccatca tccaagtata tcaagatcaa aataattata aaacatactt
1261 gtttattata atagataggt actcaaggtt agagcatatg aatagatgct gcatatgcca
1321 tcatgtatat gcatcagtaa aacccacatc aacatgtata cctatcctag atcgatattt
1381 ccatccatct taaactcgta actatgaaga tgtatgacac acacatacag ttccaaaatt
1441 aataaataca ccaggtagtt tgaaacagta ttctactccg atctagaacg aatgaacgac
1501 cgcccaacca caccacatca tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc
1561 agtaaaaccc gcatcaacat gtatacctat cctagatcga tatttccatc catcatcttc
1621 aattcgtaac tatgaatatg tatggcacac acatacagat ccaaaattaa taaatccacc
1681 aggtagtttg aaacagaatt ctactccgat ctagaacgac cgcccaacca gaccacatca
1741 tcacaaccaa gacaaaaaaa agcatgaaaa gatgacccga caaacaagtg cacggcatat
1801 attgaaataa aggaaaaggg caaaccaaac cctatgcaac gaaacaaaaa aaatcatgaa
1861 atcgatcccg tctgcggaac ggctagagcc atcccaggat tccccaaaga gaaacactgg
1921 caagttagca atcagaacgt gtctgacgta caggtcgcat ccgtgtacga acgctagcag
1981 cacggatcta acacaaacac ggatctaaca caaacatgaa cagaagtaga actaccgggc
2041 cctaaccatg gaccggaacg ccgatctaga gaaggtagag aggggggggg ggggaggacg
2101 agcggcgtac cttgaagcgg aggtgccgac gggtggattt gggggagatc tggttgtgtg
2161 tgtgtgcgct ccgaacaaca cgaggttggg gaaagagggt gtggaggggg tgtctattta
2221 ttacggcggg cgaggaaggg aaagcgaagg agcggtggga aaggaatccc ccgtagctgc
2281 cgtgccgtga gaggaggagg aggccgcctg ccgtgccggc tcacgtctgc cgctccgcca
2341 cgcatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact ctcgagaggg
2401 gtccagaggc agcgacagag atgccgtgcc gtctgcttcg cttggcccga cgcgacgctg
2461 ctggttcgct ggttggtgtc cgttagactc gtcgacggcg tttaacaggc tggcattatc
2521 tactcgaaac aagaaaaatg tttccttagt ttttttaatt tcttaaaggg tatttgttta
2581 atttttagtc actttatttt attctatttt atatctaaat tattaaataa aaaaactaaa
2641 atagagtttt agttttctta atttagaggc taaaatagaa taaaatagat gtactaaaaa
2701 aattagtcta taaaaaccat taaccctaaa ccctaaatgg atgtactaat aaaatggatg
2761 aagtattata taggtgaagc tatttgcaaa aaaaaaggag aacacatgca cactaaaaag
2821 ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc ctttagacca tgtctaactg
2881 ttcatttata tgattctcta aaacactgat attattgtag tactatagat tatattattc
2941 gtagagtaaa gtttaaatat atgtataaag atagataaac tgcacttcaa acaagtgtga
3001 caaaaaaaat atgtggtaat tttttataac ttagacatgc aatgctcatt atctctagag
3061 aggggcacga ccgggtcacg ctgcactgca ggaattcgat atcaagcttg gcactggccg
3121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
3181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
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