pHY-P43_枯草杆菌载体_质粒载体_U平台商城-普拉特泽生物

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pHY-P43

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基本信息

产品名称： pHY-P43
产品编号： P1611
规格： 10μl质粒/300μl菌液
启动子： P43 promoter
复制子： p15A ori，repB
质粒大小： 5166bp
原核抗性：氨苄青霉素Amp
克隆菌株：大肠杆菌DH5α
培养条件： 37℃，有氧，LB
表达宿主：枯草杆菌
质粒简介： pHY-P43载体是枯草杆菌表达质粒，P43启动子驱动目的基因的表达。
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质粒序列：点击查看序列

LOCUS Exported 5166 bp ds-DNA circular SYN 22-OCT-2017

DEFINITION synthetic circular DNA

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 5166)

AUTHORS .

TITLE Direct Submission

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..5166

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 7..286

/label=P43 promoter

misc_feature 290..299

/label=RBS

misc_feature 307..338

/label=MCS

CDS complement(396..1772)

/codon_start=1

/gene="Bacillus subtilis tetL"

/product="tetracycline efflux protein"

/label=TcR

/note="confers resistance to tetracycline"

/translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN

KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS

LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA

HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS

ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY

MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA

SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT

GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF

CDS complement(1979..2983)

/codon_start=1

/gene="repB"

/product="RepB replication protein"

/label=repB

/note="from Enterococcus faecalis plasmid pAM-alpha-1"

/db_xref="GI:22652809"

/protein_id="AAN03827.1"

/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY

FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI

QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV

GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN

VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS

YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"

promoter 3480..3584

/gene="bla"

/label=AmpR promoter

CDS 3585..4445

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 4610..5154

/direction=RIGHT

/label=p15A ori

/note="Plasmids containing the medium-copy-number p15A

origin of replication can be propagated in E. coli cells

that contain a second plasmid with the ColE1 origin."

ORIGIN

1 aagctttgat aggtggtatg ttttcgcttg aacttttaaa tacagccatt gaacatacgg

61 ttgatttaat aactgacaaa catcaccctc ttgctaaagc ggccaaggac gctgccgccg

121 gggctgtttg cgtttttgcc gtgatttcgt gtatcattgg tttacttatt tttttgccaa

181 agctgtaatg gctgaaaatt cttacattta ttttacattt ttagaaatgg gcgtgaaaaa

241 aagcgcgcga ttatgtaaaa tataaagtga tagcggtacc attataggta agagaggaac

301 gtacacagat ctgcaggtcg acggatcccc gggaattcct gttataaaaa aaggatcaat

361 tttgaactct ctcccaaagt tgatccctta acgatttaga aatccctttg agaatgttta

421 tatacattca aggtaaccag ccaactaatg acaatgattc ctgaaaaaag taataacaaa

481 ttactataca gataagttga ctgatcaact tccataggta acaacctttg atcaagtaag

541 ggtatggata ataaaccacc tacaattgca atacctgttc cctctgataa aaagctggta

601 aagttaagca aactcattcc agcaccagct tcctgctgtt tcaagctact tgaaacaatt

661 gttgatataa ctgttttggt gaacgaaagc ccacctaaaa caaatacgat tataattgtc

721 atgaaccatg atgttgtttc taaaagaaag gaagcagtta aaaagctaac agaaagaaat

781 gtaactccga tgtttaacac gtataaagga cctcttctat caacaagtat cccaccaatg

841 tagccgaaaa taatgacact cattgttcca gggaaaataa ttacacttcc gatttcggca

901 gtacttagct ggtgaacatc tttcatcata taaggaacca tagagacaaa ccctgctact

961 gttccaaata taattccccc acaaagaact ccaatcataa aaggtatatt tttccctaat

1021 ccgggatcaa caaaaggatc tgttactttc ctgatatgtt ttacaaatat caggaatgac

1081 agcacgctaa cgataagaaa agaaatgcta tatgatgttg taaacaacat aaaaaataca

1141 atgcctacag acattagtat aattcctttg atatcaaaat gaccttttat ccttacttct

1201 ttctttaata atttcataag aaacggaaca gtgataattg ttatcatagg aatgagtaga

1261 agataggacc aatgaatata atgggctatc attccaccaa tcgctggacc gactccttct

1321 cccatggcta ctatcgatcc aataagacca aatgctttac ccctattttc ctttggaata

1381 tagcgcgcaa ctacaaccat tacgagtgct ggaaatgcag ctgcaccagc cccttgaata

1441 aaacgagcca taataagtaa ggaaaagaaa gaatggccaa caaacccaat taccgacccg

1501 aaacaattta ttataattcc aaataggagt aaccttttga tgcctaattg atcagatagc

1561 tttccatata cagctgttcc aatggaaaag gttaacataa aggctgtgtt cacccagttt

1621 gtactcgcag gtggtttatt aaaatcattt gcaatatcag gtaatgagac gttcaaaacc

1681 atttcattta atacgctaaa aaaagataaa atgcaaagcc aaattaaaat ttggttgtgt

1741 cgtaaattcg attgtgaata ggatgtattc acatttcacc ctccaataat gagggcagac

1801 gtagtttata gggttaatga tacgcttccc tcttttaatt gaaccctgtt acattcatta

1861 ttcattacac ttcataatta attcctccta aacttgatta aaacatttta ccacatataa

1921 actaagtttt aaattcagta tttcatcact tatacaacaa tatggcccgt ttgttgaact

1981 actctttaat aaaataattt ttccgttccc aattccacat tgcaataata gaaaatccat

2041 cttcatcggc tttttcgtca tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa

2101 ggtttaattt tttatgtatt tcttttaaca aaccaccata ggagattaac cttttacggt

2161 gtaaaccttc ctccaaatca gacaaacgtt tcaaattctt ttcttcatca tcggtcataa

2221 aatccgtatc ctttacagga tattttgcag tttcgtcaat tgccgattgt atatccgatt

2281 tatatttatt tttcggtcga atcatttgaa cttttacatt tggatcatag tctaatttca

2341 ttgccttttt ccaaaattga atccattgtt tttgattcac gtagttttct gtattcttaa

2401 aataagttgg ttccacacat accaatacat gcatgtgctg attataagaa ttatctttat

2461 tatttattgt cacttccgtt gcacgcataa aaccaacaag atttttatta atttttttat

2521 attgcatcat tcggcgaaat ccttgagcca tatctgacaa actcttattt aattcttcgc

2581 catcataaac atttttaact gttaatgtga gaaacaacca acgaactgtt ggcttttgtt

2641 taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct ctcctccagt

2701 tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt ctttcgcctg

2761 tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt tcagcctttt

2821 taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc ttttctctcc

2881 atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt tcatctgaat

2941 aaatgctact attaggacac ataatattaa aagaaacccc catctattta gttatttgtt

3001 tggtcactta taactttaac agatggggtt tttctgtgca accaatttta agggttttcc

3061 aatactttaa aacacataca taccaacact tcaacgcacc tttcagcaac taaaataaaa

3121 atgacgttat ttctatatgt atcaagataa gaaagaacaa gttcaaaacc atcaaaaaaa

3181 gacacctttt caggtgcttt ttttatttta taaactcatt ccctgatctc gacttcgttc

3241 tttttttacc tctcggttat gagttagttc aaattcgttc tttttaggtt ctaaatcgtg

3301 tttttcttgg aattgtgctg ttttatcctt taccttgtct acaaacccct taaaaacgtt

3361 tttaaaggct tttaagcgtc tgtacgttcc ttaaggaatt attccttagt gctttctagg

3421 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtcc

3481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac

3541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt

3601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag

3661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg

3721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa

3781 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc

3841 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag

3901 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa

3961 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc

4021 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg

4081 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa

4141 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa

4201 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg

4261 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag

4321 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg

4381 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt

4441 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt

4501 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac

4561 gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct tgagatcgtt

4621 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggaggttttt

4681 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgca ggagcgcagt

4741 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct

4801 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac

4861 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata

4921 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa

4981 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac

5041 gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca

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