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pLVCT-tTR-KRAB
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pLVCT-tTR-KRAB
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基本信息
产品名称:
pLVCT-tTR-KRAB
产品编号:
P1569
规格:
10μl质粒/300μl菌液
质粒简介:
Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation.
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LOCUS Exported 12877 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12877)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..12877
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 6..639
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 686..811
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1304..1537
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2061..3728
/label=CAG promoter
misc_feature 3814..3932
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS 4015..4731
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 4827..5378
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 5381..5998
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
CDS 6002..6022
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6125..6319
/codon_start=1
/gene="ZNF10"
/gene_synonym="KOX1"
/product="Kruppel-associated box (KRAB) transcriptional
repression domain from the human zinc finger protein ZNF10
(Margolin et al., 1994)"
/label=KRAB
/translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
QLTKPDVILRLEKGEEPWLV"
misc_feature 6461..7049
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
protein_bind 7187..7457
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
LTR 7496..7676
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter 8507..8611
/gene="bla"
/label=AmpR promoter
CDS 8612..9472
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 9643..10231
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 10477..10806
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10657..10792
/label=SV40 ori
/note="SV40 origin of replication"
intron 11940..12005
/label=small t intron
/note="simian virus 40 (SV40) small t antigen intron"
CDS 12135..12155
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 12580..12714
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
&nbs
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